Runx1基因mRNA表达与非小细胞肺癌发生及淋巴转移的相关性研究  

Association study of mRNA expression of Runx1 gene with tumorigenesis and lymphatic metastasis in human non-small cell lung cancer

作　　者：吴卫兵[1] 陈亮[1] 王俊[1] 陈广明[1] 

机构地区：[1]南京医科大学第一附属医院心胸外科,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2009年第5期682-685,共4页Journal of Nanjing Medical University(Natural Sciences)

摘　　要：目的:探讨Runx1基因mRNA表达与人非小细胞肺癌(NSCLC)发生、发展的关系。方法:取37例NSCLC标本,1O例肺良性病变旁正常肺组织,应用逆转录-多聚合酶链反应(RT-PCR)的方法检测NSCLC中Runx1的mRNA表达水平,进行统计分析。结果:肺癌肿瘤组织中Runx1基因表达水平显著低于肺良性病变旁正常肺组织(P<0.01),肺癌组织中Runx1基因表达水平降低与淋巴结转移相关(P<0.05),与肺癌组织学类型、细胞分化程度以及患者的年龄、性别无关(P>0.05)。结论:与良性病变旁正常肺组织相比,Runx1基因的mRNA水平在肿瘤组织中表达下降,并且与淋巴结转移相关,提示其可能参与肺癌的发生发展过程。Objective：To investigate mRNA expression of human runt-related transcription factor1 （Runx1） in non small cell lung cancer（NSCLC）,and its genesis and development. Methods：The mRNA expression of Runx1 was measured by semi-quantitative RT- PCR in 37 cases of NSCLC tissues and 10 cases of normal tissues beside the lung benign lesion tissues. Results：The mRNA expression level of Runx1 gene was significantly lower in NSCLC tissues than in normal tissues （P 〈 0.01）. The mRNA expression level of Runx1 gene was significandy related to lymphatic metastasis （P 〈 0.05）. Conclusion： Compared with the control,the expression of Runx1 mRNA was dereased and was correlated with lymph node metastasis. It suggests that Runx1 should be involved in the generation and development of NSCLC.

关 键 词：RUNX1 非小细胞肺癌 肿瘤发生 淋巴转移 

分 类 号：R734.2[医药卫生—肿瘤]