ET-1在食管鳞状上皮细胞癌中的表达及其对癌细胞侵袭的影响  

Effect of endothelin-1 on invasion of squamous cell carcinoma of esophagus

作　　者：徐静[1] 张晓兰[1] 脱淼[1] 杨永洁[2] 金毅[2] 

机构地区：[1]青岛大学医学院附属医院,山东青岛266003 [2]青岛大学医学院,山东青岛266003

出　　处：《中国现代医学杂志》2009年第9期1298-1300,共3页China Journal of Modern Medicine

摘　　要：目的探讨内皮素-1(Endothelin 1,ET-1)对食管鳞状上皮细胞癌细胞侵袭作用的影响。方法用Western blotting法检测75例食管鳞癌组织及配对正常食管粘膜上ET-1的蛋白表达,并用双重ET受体抑制剂Bosentan进行干预,用Matrigel体外侵袭试验显示食管癌细胞侵袭能力。结果Western blotting法显示74.7%(56/75)的食管癌组织中有ET-1蛋白高表达;Bosentan抑制ET-1与其受体结合并降低其生物作用,显著抑制食管癌细胞的体外侵袭。结论ET-1可增强人类食管鳞癌细胞的侵袭力。[Objective] To explore the effects of endothelin-1 on invasion of squamous cell carcinoma of esophagus. [Methods] Western Blotting were employed to detect the expression of ET-1 protein in 75 samples with squamous cell carcinoma of esophagus and matched normal esophageal mucous membrane, and intervention with bosentan, a dual ET （A/B）-receptor antagonist. Matrigel invasion assays in vitro were used to show the invasive abil- ity of esophageal cancer cells. [Results] 74.7% （56/75） tumors had an overexpression of ET-1 protein by Western Blotting. Bosentan inhibited the combination between ET-1 and it＇s receptor, and degrade ET-1 activity, significantly inhibited Matrigel invasion of cancer cells in vitro. [ Conclusion] Endothelin-1 can increase the invasive ability of human esophageal cancer ceils.

关 键 词：食管肿瘤 内皮素1 侵袭 

分 类 号：R735.1[医药卫生—肿瘤]