siRNA对SOM基因表达的抑制研究  

Inhibition effects of siRNA on expression of somatostatin gene

作　　者：朱晓燕[1] 岳保红[2] 张钦宪[1] 

机构地区：[1]郑州大学医学院组胚教研室,河南郑州450052 [2]郑州大学第一附属医院检验科,河南郑州450052

出　　处：《中国现代医学杂志》2009年第9期1308-1310,共3页China Journal of Modern Medicine

基　　金：河南省重大科技攻关基金(No:0222031300)

摘　　要：目的研究siRNA对生长抑素SOM基因表达的抑制作用。方法根据SOM全长cDNA序列设计和合成RNA干扰的靶序列,应用RiboMAXT7体外转录合成SiRNA,并转染胃癌细胞系SGC-7901,经RT-PCR,Western blot检测SOM mRNA及蛋白质的表达水平。结果siRNA可以特异性抑制SOM基因的表达。结论通过体外合成siRNA可以特异性抑制SOM基因的表达,为筛选抑制SOM基因表达的有效序列研究奠定了基础。[Objective] To study the inhibition effects of siRNA on expression of somatostatin gene. [Methods] According to the gene sequence of SOM in the Genbank, we designed the targeted siRNA templates and constructed the siRNA using the RiboMAXT7 in vitro. The siRNA was transfected into gastric cancer cell line SGC-7901 and the inhibitory effect was detected by RT-PCR and Western blot. [Results] siRNA inhibited SOM gene expression. [Conclusion] siRNA was generated in vitro and it inhibited SOM gene expression. Our research provides basic research for selecting efficient sequence of suppressing SOM gene expression.

关 键 词：RNA干扰 生长抑素基因 

分 类 号：R735.2[医药卫生—肿瘤]