NS398对宫颈癌Hela细胞bcl-2表达的影响  

Effects of NS398 on the expression of bcl-2 in cervical cancer Hela cell

作　　者：邹明英[1] 

机构地区：[1]郴州市第一人民医院,湖南郴州423000

出　　处：《中国现代医学杂志》2009年第9期1380-1383,共4页China Journal of Modern Medicine

摘　　要：目的探讨NS398对宫颈癌Hela细胞生长及bcl-2基因和蛋白表达的影响。方法用不同浓度的NS398(0、25、50、75和100μmol/L)处理宫颈癌Hela细胞不同时间,采用MTT比色法分析细胞生长抑制率。用不同浓度NS398处理Hela细胞24h,RT-PCR检测COX-2和bcl-2 mRNA表达的变化,Western blot检测COX-2和bcl-2蛋白表达的变化。结果随着NS398浓度及处理时间的增加,宫颈癌Hela细胞生长抑制率升高;随着NS398剂量的增加,COX-2和bcl-2 mRNA及蛋白的表达水平逐渐减少。结论NS398可呈剂量和时间依赖性抑制Hela细胞的生长,呈浓度依赖性减少COX-2和bcl-2 mRNA及蛋白的表达。NS398可能通过抑制COX-2和bcl-2的表达,抑制宫颈癌Hela细胞的生长。[Objective] To study the effects of NS398 on the cell growth and expression of bcl-2 in cervical cancer Hela cell. [Methods] After Hela cells were treated with NS398 （0, 25, 50, 75 and 100 μmol/L） for 12, 24, 48 or 72 hours, the growth inhibitory rate of cell was evaluated by MTT assay. The mRNA expressions of COX-2 and bcl-2 were detected by RT-PCR. Protein expressions of COX-2 and bcl-2 were detected by Western blot. [Resuits] The growth inhibition rate was elevated by NS398 with increased of concentration and time in Hela cell lines. RT-PCR and Western blot revealed that NS398 decreased the mRNA and protein expressions of COX-2 and bcl-2 with increased of concentration. [Conclusions] NS398 inhibits Hela cell growth in a dose-and time-dependent manners. The expressions of COX-2 and bcl-2 are decreased by NS398 in a dose-dependent manner. NS398 can inhibit proliferation of cervical cancer Hela cell line in vitro, which may contributed to decrease the expressions of COX-2 and bcl-2.

关 键 词：NS398 HELA细胞 环氧化酶-2 BCL-2 

分 类 号：R737.33[医药卫生—肿瘤]