内毒素诱导的大鼠葡萄膜炎细胞凋亡的研究  

Endotoxin-induced cell apoptosis in rats uveitis

作　　者：张玮[1] 卢弘[1] 华文[1] 李学东[1] 

机构地区：[1]首都医科大学附属北京朝阳医院眼科,北京100020

出　　处：《眼科研究》2009年第5期374-379,共6页Chinese Ophthalmic Research

摘　　要：目的探讨大鼠内毒素诱导的葡萄膜炎(EIU)中葡萄膜和视网膜组织浸润细胞的凋亡,及Fas-FasL的表达与细胞凋亡的相关性。方法注射霍乱弧菌内毒素诱发大鼠EIU模型,内毒素注射后6、12、18、24、48h进行临床观察评分并行组织病理学检查。应用TUNEL法检测葡萄膜和视网膜中的凋亡细胞,免疫组织化学法检测Fas和FasL在葡萄膜和视网膜中的表达和变化。结果实验组大鼠均发生了EIU;葡萄膜和视网膜组织浸润细胞中可见TUNEL染色阳性凋亡细胞,18h达高峰,以虹膜和睫状体最为明显,凋亡阳性细胞数与正常对照组相比,差异有统计学意义(P<0.01);实验各组中均可见Fas表达,实验各组中FasL表达显著增强,与正常对照组相比差异有统计学意义(P<0.01)。Fas和FasL的表达与凋亡之间呈正相关(r=0.923,0.807,P<0.01)。结论EIU中葡萄膜和视网膜的浸润细胞存在凋亡现象,凋亡参与了炎症的迅速消退,Fas和FasL的高表达可能与内毒素诱导的葡萄膜炎眼组织的细胞凋亡有关。Objective Researches showed that the cellular apoptosis is an important mechanism of regression of endotoxininduced uveitis （EIU） , and the apoptosis is associated with the expression of Fas antigen （Fas） and Fas ligand（ FasL）. Our study was to observe the cellular apoptosis of urea and retina in endotoxin-induced uveitis model and the relevance of Fas/FasL with the cell apopstosis. Methods The models of severe endotoxin-induced uveitis were induced in rats by the intraperitoneal and plantar injection of cholera vibrio lipopolysaccharide （LPS） ＋ phosphate buffer saline （PBS） ＋ complete Freund ＇ s adjuvant （CFA） in 25 Wistar rats,and PBS injection was used as control in 5 rats. The clinical manifestation of eyes was observed under the slim-lamp and opthalmoloscope at a two-hour interval and scored based on the Hoekzema＇ s criteria. The rats were sacrificed at 6,12,18,24 and 48 hours after injection of endotoxin respectively for the histological examinations. Cellular apoptosis was evaluated by TUNEL assay and expressions of Fas and FasL in urea and retiaa was detected by immunohistochemistry. Results The corneal edema, anterior chamber exudation and iris edema were found at 10 hours after injection in model group and the inflammation score was gradually increased with lapse of time, and no inflammatory reaction was found in PBS injection group. Infiltration of neutrophils,monocytes and lymphocytes into the urea and retina was noted by routine histological examination. A lots of TUNEL positive cells were seen in the urea and retina at various time points in EIU model group compared with control groop（P 〈0. 01 ）. Many apoptotic cells were exhibited mainly in the iris and ciliary body with the peak value at 18 hour after LPS injection. Positive staining for Fas protein was not found in normal group, but Fas was expressed in the cells of urea and retina in model group rather than control group and showed a gradually increasing tendency from 6 to 18 hour after injection of endotoxi

关 键 词：内毒素 葡萄膜炎 凋亡 FAS FASL 

分 类 号：R773[医药卫生—眼科]