淫羊藿总黄酮及其含药血清对成骨细胞增殖及功能表达的影响  

Effect of total flavonoids of Epimedium sagittatum and its metabolites on osteoblast proliferation and function expression

作　　者：武密山[1] 赵素芝[2] 李恩[3] 白霞[1] 郝秀红[2] 

机构地区：[1]河北医科大学中医学院,河北石家庄050091 [2]石家庄市桥东区医院,河北石家庄050041 [3]河北医科大学生化教研室,河北石家庄050091

出　　处：《中国药理学通报》2009年第5期613-616,共4页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No30472200);河北省引进留学人员经费资助项目(No200828)

摘　　要：目的研究淫羊藿总黄酮及其含药血清对体外培养大鼠成骨细胞(ROB)增殖和功能表达的影响。方法将淫羊藿总黄酮(TFE)以0.2、2、20、100、200 mg.L-15种质量浓度、含淫羊藿总黄酮大鼠血清(SRAT)以2%、4%、8%、16%等4种体积浓度分别加入新生大鼠颅骨成骨细胞培养液中,研究其对细胞增殖和功能表达的影响。细胞增殖采用MTT法进行分析,细胞功能表达是于培养d 5、10、15、20分别检测碱性磷酸酶活性(比色法)、骨钙素分泌量(放射免疫法)、钙盐沉积量(比色法)和矿化结节(组织化学法)等。结果TFE5种质量浓度均对ROB细胞增殖和分化无影响,2%和4%SRAT则表现出强烈的刺激细胞增殖活性并提高碱性磷酸酶活性、骨钙素分泌量、矿化结节数和钙盐沉积量。结论淫羊藿治疗骨质疏松的直接物质基础可能并非TFE,而与其经口给予后吸收入血的TFE代谢产物及其诱生物有关。血清中的TFE代谢产物可促进细胞的功能表达增殖。Aim To investigate the effect of total flavonoids of Epimedium sagittatum （TFE）and its metabolites on the proliferation and function expression of newborn rats calvarial osteoblasts （ROB）. Methods rIFE was supplemented into the culture medium of ROB at 0.2, 2, 20, 100 and 200mg·L^-1 respectively. The serum of rats administered TFES （SRAT） was also added into the medium in a parallel treatment at 2% , 4% , 8% and 16% respectively. Their effects on cell proliferation and function expression were studied by MTT and the analysis of osteogenic differentiation marks. Results TFE had no effect on cell proliferation and function expression at any concentration. However, 2% and 4% SRAT stimulated cell proliferation and, 4% SRAT promoted the maturation and func- tion of osteoblast by improving the alkaline phosphatase （ALP） activity, bone gla protein （BGP） secretion, calcium deposition and the number of mineralized nodular structures. Conclusions The effective substance of herba epimedii treating on osteoporosis is not TFE itself but certain of metabolites derived from TFE and the products induced by these metabolites in serum. The metabolites in serum of TFE may enhance the cell function expression and proliferation.

关 键 词：淫羊藿总黄酮 含药血清 成骨细胞 功能表达 增殖 

分 类 号：R-332[医药卫生] R284.1