五倍子牙周缓释凝胶对LPS介导HPDLFs分泌MMP-3的影响  

Effects of periodontal sustained-released gel of Chinese nutgall on the expression of MMP-3 in human periodontal ligament fibroblasts induced by lipopolysaccharide

作　　者：祝磊[1] 唐荣银[1] 张海欧[1] 张安生[1] 杨素芳[2] 

机构地区：[1]第四军医大学口腔医学院,陕西西安710032 [2]解放军总政治部门诊部口腔科,北京100011

出　　处：《牙体牙髓牙周病学杂志》2009年第5期271-274,共4页Chinese Journal of Conservative Dentistry

摘　　要：目的:探讨五倍子牙周缓释凝胶对内毒素(LPS)介导人牙周膜成纤维细胞(HPDLFs)分泌基质金属蛋白酶-3(MMP-3)的影响。方法:体外培养HPDLFs,运用ELISA分别检测在LPS作用下以及在LPS加不同时间五倍子凝胶释放液作用下HPDLFs分泌MMP-3的量。结果:与空白对照组相比,50μg/mL的LPS即能明显促进HPDLFs分泌MMP-3(P<0.05),100μg/mL的LPS促进作用更显著;加入五倍子凝胶释放液后,各时间点凝胶释放液组MMP-3的量均明显低于LPS组(P<0.05),而且,各时间点凝胶释放液组之间亦有显著性差异(P<0.05),其中,2h释放液组MMP-3最低,相对于LPS组,对MMP-3的抑制率为75.77%,随着释放时间的延长,抑制作用逐渐减弱,但第7天释放液仍有32.26%的抑制率。结论:五倍子牙周缓释凝胶可有效抑制LPS介导HPDLFs分泌MMP-3,抑制作用可持续7d。AIM： To detect the influence of a periodontal sustained - released gel of Chinese nutgall on the secretion of MMP - 3 in human periodontal ligament fibroblasts （ HPDLFs ） induced by lipopolysaeeharide （ LPS ） . METHODS： HPDLFs were cultured in vitro and treated with LPS or with LPS ＋ Chinese nutgall solutions released from the gel at different time points. The amounts of MMP - 3 secreted by HPDLFs were detected by ELISA. RESULTS： When the groups treated by LPS were compared with the blank group, significant increases（P 〈0.05） in MMP - 3 level were observed in the groups treated by LPS of the concentration from 50 to 1001μg/mL. After treated with Chinese nutgall solutions released from the gel, the amount of MMP - 3 of all the groups were significantly lower than that of the LPS group （ P 〈 0.05 ）. Significant differences were found among all the groups, the amount of MMP - 3 secretion of HPDLFs treated with the solution released from the gel in the first 2 hours was the lowest. Compared with the LPS group, the inhibition ratio of MMP -3 of this solution was 75.77%. But with the extending of releasing time, the inhibitory action was weakening gradually, however, the inhibition ratio still remained as high as 32.26% on the 7th day. CONCLU- SION： The periodontal sustained -released gel of Chinese nutgall can significantly inhibit the secretion of MMP -3 in human periodontal ligament fibroblasts induced by LPS, and the inhibitory action can last 7 days.

关 键 词：五倍子牙周缓释凝胶 人牙周膜成纤维细胞 基质金属蛋白酶一3 内毒素 

分 类 号：R781.4[医药卫生—口腔医学]