不同剂量地塞米松对小鼠胚胎腭裂发生的影响  

Effect on incidence of cleft palate interfered by different dose dexamethasone in mice embryo

作　　者：何苇[1,2] 卢胜军[1] 李承浩[1] 蒙田[1,2] 周京琳[1] 石冰[1,2] 

机构地区：[1]口腔疾病研究国家重点实验室,四川大学,四川成都610041 [2]四川大学华西口腔医院唇腭裂外科,四川成都610041

出　　处：《国际口腔医学杂志》2009年第3期270-272,共3页International Journal of Stomatology

基　　金：国家自然科学基金资助项目(30530730)

摘　　要：目的确立地塞米松诱导小鼠腭裂模型的最佳剂量,为进一步相关实验提供可靠模型。方法分别将特定孕期的C57BL/6J雌鼠随机分为5组,即对照组和实验Ⅰ～Ⅳ组。E10-E12时,每天早上8时给予对照组每只孕鼠腹腔注射生理盐水0.1mL,实验Ⅰ、Ⅱ、Ⅲ、Ⅳ组孕鼠分别腹腔注射地塞米松12.0、6.0、3.2、1.6mg/kg。于E17.5时处死孕鼠,称量并计算孕鼠和胚胎的体重,记录胚胎吸收数及胚胎腭裂数。结果实验Ⅲ、Ⅳ组孕鼠与对照组孕鼠的体重的差异具有统计学意义(P<0.05),但其胚胎吸收数和胚胎腭裂数的差异无统计学意义(P>0.05);实验Ⅰ、Ⅱ组与对照组孕鼠胚胎腭裂发生率的差异具有统计学意义(P<0.05);实验Ⅰ组与对照组孕鼠胚胎吸收率的差异具有统计学意义(P<0.05);各组间胚胎体重的差别无统计学意义(P>0.05)。结论6.0mg/kg地塞米松是建立C57BL/6J胎鼠腭裂模型的适宜剂量。Objective To define the optimal dose of dexamethasone induced cleft palate in C57BL/6J mice and to provide a reliable model of cleft palate for further study. Methods Pregenant mice at specific period were divided into five groups randomly, that were control group and experimental groups of Ⅰ,Ⅱ,Ⅲ, Ⅳ The mice of the control group were injected sodium chloride 0.1 mL and the mice of experimental Ⅰ , Ⅱ,Ⅲ,Ⅳ groups were injected dexamethasone of 12.0, 6.0, 3.2, 1.6 mg/kg respectively at 8：00 am on embryo 10 to embryo 12. To weigh pregnant mice and embryos and record the number of embryonic resorption and cleft palate after pregnant miee were killed on embryo 17.5. Results Weight of pregnant mice was significantly different（P〈0.05）, but the number of embryonic resorption and cleft palate had no significant difference between control group and experimental Ⅲ,Ⅳ groups （P〉0.05）. Incidence of embryonic deft palate had significant difference between control group and experimental Ⅰ, Ⅱgroups （P〈0.05）. However, incidence of embryonic resorption increased significantly in experimental Ⅰ group （P〈0.05）. There was no difference in embryonic weight among each group （P〉0.05）. Conclusion 6.0 mg/kg is the optimal dose of dexamethasone to induce cleft palate in C57BL/6J mice.

关 键 词：腭裂 地塞米松 小鼠 

分 类 号：R782[医药卫生—口腔医学]