硫代乙酰胺建立大鼠急性肝损伤模型探讨  

A Rat Model of Acuted Liver Injury Induced by Thioacetamide

作　　者：魏新智[1] 付勇强[1] 王珲[1] 王英[1] 孙科峰[1] 尤春来[1] 

机构地区：[1]辽宁中医药大学附属医院中医药实验中心,辽宁沈阳110032

出　　处：《临床医学工程》2009年第5期48-49,共2页Clinical Medicine & Engineering

摘　　要：目的探讨用硫代乙酰胺(Thioacetamide,TAA)制作大鼠急性肝损伤模型时选择TAA的合适剂量、指标检测的最佳时间点以及大鼠的适宜性别。方法一次性腹腔注射不同剂量的TAA制作大鼠急性肝损伤模型,检测血清谷草转氨酶(AST)、谷丙转氨酶(ALT)值及肝脏病理学变化。结果TAA200mg/kg的剂量组血清ALT、AST值显著升高,病理学显示肝细胞变性坏死,而且肝脏病理变化均一。各组雌性鼠的各项指标均不如雄性鼠明显。结论在本实验条件下TAA200mg/kg的剂量最佳,雄性鼠优于雌性鼠,给药后24小时急性肝损伤最明显。Objective A rat model of acuted liver injury induced by thioacetamide was esblished, and to study the best dose of thioace tamide and time point and the most appropriate gender of the rats in this model. Methods The model of acute liver injury was induced by in traperitoneal injection of different dose of thioacetamide, measuring the values of ALT and AST in serum and the changes of liver morphology. Results The serum ALT and AST values were significantly higher in Thioacetamide 200 mg / kg group and histology showed that stem cell de generation and necrosis. And the liver change was uniform. Conclusion In this experimental condition TAA 200 mg/kg is the best dose, the male rats is better than the female and after 24 hours of injection thioacetamide is the best time point for the acute live injury.

关 键 词：硫代乙酰胺 大鼠 急性肝损伤 

分 类 号：R575[医药卫生—消化系统]