Study on the Developmental Changes of Muscular GHR mRNA Expression in Sheep  

绵羊肌肉生长激素受体基因表达的发育性变化研究(英文)

作　　者：黄治国[1,2] 谢庄[2] 

机构地区：[1]四川理工学院酿酒生物技术及应用四川省重点实验室,四川自贡643000 [2]南京农业大学动物科技学院,江苏南京210095

出　　处：《Agricultural Science & Technology》2009年第1期93-96,共4页农业科学与技术（英文版）

基　　金：Supported by Doctoral Fund of Xinjiang Production and Construction Corps of CPLA (2003-02)~~

摘　　要：[ Objective] The study aimed to explore the expression of muscular growth hormone receptor gene （GHR） in sheep at the early stage of growth and development. [Method] The GHR mRNA expression levels in longissimus dorsal muscles of male Kazak sheep and Xinjiang fine wool sheep with different ages were quantitatively analyzed by real time PCR. [ Result] Sheep GHR mRNA expression level in longissimus dorsal muscle increased firstly followed by decline, and then kept steady until the end of the experiment, with the expression peak appearing on postnatal day 30. The GHR mRNA expression level of Kazak sheep was extremely lower than that of Xingjiang fine wool sheep from 2 to 90 days old （ P 〈0.01 ）. E Conclusionl Both age and breed had great effects on the expression of muscular GHR gene in sheep.1材料与方法1．1试验动物选取新疆维吾尔自治区石河子市紫泥泉种羊场的2、30、60、90和120日龄的雄性哈萨克羊和新疆细毛羊各6只（120日龄的只有新疆细毛羊），共计54只，测体重后屠宰，采取背最长肌，立即置于液氮中速冻，-70℃保存。

关 键 词：SHEEP MUSCLE Growth hormone receptor gene （GHR） EXPRESSION 

分 类 号：S826[农业科学—畜牧学]