磁性纳米粒标记的神经干细胞不同移植途径对大鼠脑损伤治疗的实验研究  

Contrast research on traumatic brain injury of rats by different transplanting ways of Nanometer SPIO-labeled neural stem cells

作　　者：黎国雄[1] 王传湄 赵洪洋[3] 龚利平[1] 田洪涛[3] 

机构地区：[1]广东省深圳市西乡人民医院神经外科,广东深圳518102 [2]广东省深圳市宝安区人民医院,广东深圳518101 [3]华中科技大学同济医学院附属协和医院,湖北武汉430022

出　　处：《中国现代医学杂志》2009年第8期1134-1138,共5页China Journal of Modern Medicine

摘　　要：目的探讨脑损伤后不同移植途径神经干细胞的迁移及分布情况并进行对比研究。方法体外培养的胚胎神经干细胞,采用Feeney自由落体脑创伤模型制成大鼠脑损伤模型,超顺磁性氧化铁纳米颗粒(SPIO)标记神经干细胞,并将其分别经枕大池穿刺注射入脑损伤大鼠蛛网膜下腔的脑脊液中及经立体定向脑内注射移植,分别进行大鼠运动功能缺失的神经功能评价、磁共振示踪及大鼠脑切片普鲁士蓝染色。结果接受神经干细胞移植的两组大鼠神经运动功能评分均较损伤对照组明显提高(P<0.05),两移植组大鼠神经运动功能评分无明显差别。接受神经干细胞移植的两组大鼠神经运动功能评分均较损伤对照组明显提高(P<0.05),两移植组大鼠神经运动功能评分无明显差别。标记组移植经枕大池移植的神经干细胞即刻的MRI即可观察到大鼠脑内的移植标记细胞,移植后2周脑挫伤即可见到低信号影,组织切片的普鲁士蓝染色与MRI结果相符。结论经枕大池移植的神经干细胞具有远距离迁移能力,并能像脑内移植一样明显有助于大鼠神经运动功能的恢复。用MRI活体示踪移植磁化标记神经干细胞在TBI模型大鼠脑内迁移和分布是可行的。[Objective] To investigate the feasibility and the different effects on the repair of adult rats experiment traumatic brain injury （TBI） when Nanometer SPIO-labeled neural stem cells （NSCs） were injected into subarachnoid space by eisterna magna and into the brain directly by stereotaxis. [Methods] Neural stern ceils （NSCs） derived from the cortex of rat embryos were injected into the rats cisterna magna and into the brain direcdy by stereotaxis. MR scanner was used to monitor the migration of transplanted ceils. The neurological motor function scores of rats were assessed at pre-injury 24 h, post-injury 24 hand after 1 and 2 weeks. Prussian blue staining were performed to study migration and functional differentiation of NSCs. [Results] There were significant improved neurologic almost or function scores in brain injured rats that received NSCs injection. There were no significant difference between the two transplanting ways. The implanted labeled cells were visible on MR images as low signal intensity area in the injected ventricles of the receptor rats immediately. The low signal intensity was observed in the subcortical infarct lesion after 14 days. MR scanning results were confirmed by Prussian blue staining of histological sections. [Conclusion] Neural stem cells have the potential to migrate into injured brain via subarachnoid space and improve the injured neurological motor function of rats liking transplanting directly by stereotaxis. NSCs labeled with SPIO Nanometer could migrate into the lesions after transplanted into the Subarachnoid space. MR scanning is useful and noninvasive to tracking the location and distribution of the implanted cells.

关 键 词：神经干细胞 移植 创伤性脑损伤 蛛网膜下腔 动物实验模型 

分 类 号：R-332[医药卫生]