三种实验方法诊断细菌性阴道病的对比研究  

A Comparative Study of Three Techniques for Diagnosis of Bacterial Vaginosis

作　　者：曹晓丽[1] 皮兰敢[1] 

机构地区：[1]湖南省郴州市第一人民医院检验科,423000

出　　处：《医学理论与实践》2009年第5期510-512,共3页The Journal of Medical Theory and Practice

摘　　要：目的:两种试剂盒的测定与传统的Amsel法在诊断细菌性阴道病的比较评价。方法:收集阴道分泌物异常的白带标本分别为80例和459例,用Amsel法、珠海丽珠试剂盒测定方法、中山天洋试剂盒测定方法进行比较。结果:用珠海丽珠试剂盒测定的80例中,40例用Amsel法被确诊为BV的患者中,丽珠阳性34例,阴性6例,40例用Am-sel法被确诊为BV阴性的患者中,丽珠阴性36例,阳性4例,丽珠测定灵敏度85%,特异度为90%,假阳性率为10%,假阴性率为15%,准确度为87.5%,P>0.05;用中山天洋试剂盒测定的459例中,115例用Amsel法被确诊为BV的患者中,中山天洋阳性82例,阴性33例,344例用Amsel法被确诊为BV阴性的的患者中,中山天洋阴性160例,阳性184例。灵敏度71.3%,特异度为46.5%,假阴性率为28.7%,假阳性率为53.5%,准确度为52.7%,P<0.001。结论:诊断细菌性阴道炎(BV),选择联合检测BV的试剂盒,进行使用前试验比对是很重要的环节,好的检测试剂盒可为临床提供有价值的诊断。Objective： Comparison and evaluation of two diagnostic kits with the traditional Amsel method in diagnosis of bacterial vaginosis （BV）. Methods：Collection of 80 and 459 samples respectively, of abnormal vaginal discharge from the leukorrhea patients, followed by comparative studies of Amsel method with Li-zhu kit method and Zhong-shan Tian-yang kit method, respectively. Results：IN the samples of 80 with Li-zhu kit, 40 BV-positive cases determined with Amsel method were confirmed 34 BV-positive and 6 BV-negative cases by Li-zhu kit analysis. Whereas 40 BV-negative cases determined with Amsel method were confirmed 36 BV-negative and 4 BV-positive cases by Li-zhu kit analysis. Suggesting the sensitivity of 85%, specificity of 90%, false positiverate of 10%, false negative rate of 15% and accuracy of 87. 50%, P〉0. 05, by Li-zhu kit analyses. On the other hand, in the samples of 459 with Zhong-Shan Tian-yang kit, 115 BV-positive cases determined with Amsel method were confirmed, by Zhong-shan Tian-yang kit, 82 BV-posi- tive and 33 BV-negative cases. Whereas 344 BV-negative cases determined with Amsel method were confirmed 160 BV- negative and 184 BV-positive by Zhong-shan Tian-yang kit analysis. Suggesting the sensitivity of 71.3%, specificity of 46. 5 %, false negative rate of 28. 7%, false positive rate of 53. 5% and accuracy of 52. 7%, P〈0. 001, by Zhong-shan Tian-yang kit analyses. Conclusion： It is important to select the combination kits for BV diagnosis and to perform a comparison tests prior to clinical diagnosis. Diagnosis of bacterial vaginosis, it is critical to choose a reliable diagnostic kit in order to provide clinical diagnostic values.

关 键 词：细菌性阴道病 丽珠检测试剂盒 中山天洋检测试剂盒 

分 类 号：R771.31[医药卫生—眼科] R446[医药卫生—临床医学]