血管抑素基因对人胰腺癌细胞PC3裸鼠原位移植瘤模型生长和转移的影响  

Effect of angiostatin gene on growth and metastasis of pancreatic carcinoma strain PC-3 orthotopic implantation model in mice

作　　者：黄春锦[1] 杜卫东[1] 沈达明[1] 袁祖荣[1] 乔伟伟[2] 唐健雄[1] 程爱群[1] 张赣生[3] 王一倩[3] 于晓峰[3] 竺越[3] 

机构地区：[1]上海市华东医院普外科,200040 [2]复旦大学动物实验部 [3]上海市华东医院消化科,200040

出　　处：《中国实用医药》2009年第14期1-2,共2页China Practical Medicine

基　　金：上海市自然科学基金资助(项目编号:04Z14045)

摘　　要：目的研究血管抑素基因对胰腺癌原位移植瘤模型裸鼠肿瘤生长和转移的影响。方法微量注射器向胰腺包膜下人胰腺癌细胞PC3(2×106个细胞),建立裸鼠人胰腺癌细胞PC3原位移植瘤模型。观察血管抑素基因治疗组裸鼠的原位肿瘤体积,各脏器肿瘤转移情况。免疫组化法检测肿瘤微血管密度(MVD)。结果血管抑素基因治疗组原位肿瘤体积小于对照组,肿瘤腹膜转移、肝转移以及其他脏器转移的发生率较低,腹水的发生率亦较低,免疫组化检查结果显示血管抑素基因治疗组裸鼠肿瘤组织中MVD明显低于其他各组(P<0.05)。结论血管抑素基因对胰腺癌原位移植瘤模型裸鼠肿瘤生长和转移有抑制作用。Objective The purpose of the experiment is to study the effect of angiostatin gene on growth and metastasis of pancreatic carcinoma strain PC-3 orthotopic implantation model in mice establish. Methods The orthotopic implantation model was established by subcapsular implantation of human pancreatic carcinoma strain PC-3 （2 × 10^6 cells） into pancrean of nude mice using microsyringe. Two weeks after implatation and gene therapy, the mice were sacrificed and the tumor sizes measured and the presence of metastasis re- corded. The microvasular density （ MVD ） was examined by immunohistochemical staning. Result Compared with the untreated controls, growth of the orthotopic implantated tumor was significantly reduced in size in mice with angiostatin gene therapy, tumor metastasis was markedly inhibited, and the MVD was also decreased. Conclusion Angiostatin gene therapy has strong inhibitory effect both on tumor growth and metastasis of pancreatic carcinoma in nude mice.

关 键 词：胰腺肿瘤 细胞株 动物模型 血管抑素 基因治疗 

分 类 号：R735.9[医药卫生—肿瘤]