甘氨酰谷氨酰胺在大鼠离体心脏缺血/再灌注损伤中的保护作用研究  

Protection of Glycyl-L-Glutamine against myocardial ischemia-reperfusion injury in the isolated rat heart

作　　者：戚仁斌[1] 熊怡[1] 陆大祥[1] 王彦平[1] 付咏梅[1] 

机构地区：[1]暨南大学医学院病理生理学教研室,广东广州510632

出　　处：《中国病理生理杂志》2009年第5期853-858,共6页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30470718)

摘　　要：目的:研究甘氨酰谷氨酰胺对缺血再灌注大鼠离体心脏的保护作用。方法:应用Langendorff离体心脏灌注系统建立心肌缺血再灌注模型。30只SD雄性大鼠随机分为正常对照组(control)、甘氨酰谷氨酰胺对照组(Gly-Gln)、缺血再灌注组(I/R)、缺血/再灌注+甘氨酰谷氨酰胺组(I/R+Gly-Gln)。I/R组及I/R+Gly-Gln组分别灌注30min后,全心停灌20min,再灌注40min,I/R+Gly-Gln组于再灌注时在灌流液中加入Gly-Gln;正常对照组连续灌流90min,Gly-Gln对照组灌流液中加入Gly-Gln。记录各组灌注时,左室舒张末压(LVEDP)、左室发展压(LVDP)、左室压力最大变化速率(±dp/dtmax)、心率(HR)及心肌细胞单相动作电位(MAP);同时在相应的时点分别测定冠脉流出液中的乳酸脱氢酶(LDH)、肌酸激酶(CK)活性。结果:离体大鼠心脏缺血20min,再灌注40min,导致严重的心功能抑制,表现为LVEDP升高,LVDP、±dp/dtmax降低;再灌注液中加入Gly-Gln后,LVEDP降低,LVDP、±dp/dtmax明显升高(P<0.01)。I/R+Gly-Gln组冠脉流出液中LDH、CK活性明显低于I/R组(均P<0.01)。结论:Gly-Gln能有效减轻缺血再灌注引起的左室功能下降,减少心肌细胞LDH、CK的释出,表明Gly-Gln对缺血再灌注损伤的大鼠离体心脏具有保护作用。AIM ： To study the protection of Glycyl - L - Glutamine（ Gly - Gin） against myocardial ischemia/ reperfusion（I/R） injury in the isolated rat heart. METHODS： A model of myocardial ischemia -reperfusion injury was established with a Langendorff apparatus. Thirty male SD rats were randomly divided into four groups ： control group, Gly - Gln group, I/R group and I/R ＋ Gly - Gin group. Both I/R and I/R ＋ Gly - Gin group were pre - perfused for 30 min, followed by 20 rain ischemia and 40 min reperfusion. During reperfusion I/R ＋ Gly -Gln group was perfused with Gly - Gin perfusate. Control group was kept perfused for 90 min. Gly - Gln group Gly - Gin perfusate was also kept perfused for 90 min. The left ventricular end - diastolic pressure （ LVEDP）, left ventricular developed pressure （ LVDP）, ±dp/dtmax, heart rate （ HR）, monophasic action potentials（MAP） was measured during perfusion. The coronary effluent fluid was collected at different certain times. The activities of lactic dehydrogenase （LDH） and creatine kinase（CK） were determined. RESULTS： The isolated rat heart function decreased severely after 20 min ischemia and 40 min reperfusion（I/R） ： the LVEDP increased and the LVDP, ± dp/dtmax decreased. But the LVEDP decreased and the LVDP, ±dp/dtmax increased in I/R ＋ Gly -Gln group compared with I/R group. Moreover, the activities of LDH and CK in the coronary effluent fluid decreased remarkably in I/R ＋ Gly - Gin group compared with I/R group. CONCLUSION： Gly - Gin can play a protective role against myocardial I/R injury in isolated rat hearts via maintaining the left ventricular function and decreasing the release of LDH and CK.

关 键 词：甘氨酰谷氨酰胺 缺血/再灌注损伤 离体心脏 

分 类 号：R363[医药卫生—病理学]