姜黄素抑制小鼠矽肺纤维化  

Curcumin inhibits SiO_2-induced pulmonary fibrosis in mice

作　　者：江振友[1] 岳磊[1] 卢燕茹[1] 董军[2] 戴国奎 杨春华[4] 卢业成[5] 

机构地区：[1]暨南大学医学院微生物学与免疫学教研室 [2]暨南大学医学院病理生理学教研室,广东广州510632 [3]广州市干部疗养院检验科,广东广州510530 [4]暨南大学附属第一医院校医门诊部,广东广州510632 [5]广州市第八人民医院,广东广州510060

出　　处：《中国病理生理杂志》2009年第5期976-979,共4页Chinese Journal of Pathophysiology

基　　金：广东省中医药局建设中医药强省科研资助项目(No.1060117);暨南大学国家中医药管理局病理生理重点实验室开放基金资助项目(No.51206011);广东省科技计划资助项目(No.83057)

摘　　要：目的:建立小鼠矽肺动物模型,探讨姜黄素在减轻小鼠矽肺纤维化中的作用。方法:用手术方法暴露小鼠气管,定量注射二氧化硅(SiO2)悬液建立矽肺模型。建模2周后将小鼠随机分为模型组、低剂量组、中剂量组和高剂量组分别给药,给药2周以及6周后处死动物,对肺组织行病理组织学检查。结果:建立了矽肺的纤维化模型。与模型组相比,给药2周后,高剂量组矽结节大小及数量显著减小(P<0.05);给药6周后,模型组以成纤维细胞为主,同时夹杂少量的胶原纤维,而给药组仍以巨噬细胞和淋巴细胞为主的细胞性矽结节为主。结论:姜黄素能抑制矽肺纤维化进程。AIM： To establish an animal model and to investigate the effect of cureumin on SiO2 - induced pulmonary fibrosis in mice. METHODS： Animal model was established by direct tracheal instillation of silica into rat lungs through tracheostomy. Two weeks after tracheostomy these mice were divided into four groups, which were poured into curcumin at different concentrations （high, medium, low） through stomach. In .control group, distilled water is used instead of curcumin. After 2 weeks and 6 weeks, the animals were killed and histopathological test was performed on lung. RESULTS： The silicosis model was established. Two weeks after treatment, the numbers and size of the nodules in high dosage group were much less and smaller than those in control group （ P 〈 0. 05 ）. Six weeks after treatment, the nodules in control group were mainly consisted of fibroblast mixed with a small amount of collagen fiber, whereas nodules in experimental group were made up of macrophages. CONCLUSION ： Cureumin inhibits the process of silicotic fibrosis.

关 键 词：矽肺 姜黄素 纤维化 

分 类 号：R735.7[医药卫生—肿瘤]