人类卵母细胞及植入前胚胎中母性效应基因Mater mRNA的表达  

Expression of Mater mRNA in human oocytes and preimplantation embryos

作　　者：张莹[1] 刘平[1] 张秋芳[1] 黄锦[1] 乔杰[1] 张小为[1] 

机构地区：[1]北京大学第三医院生殖中心,北京100083

出　　处：《现代妇产科进展》2009年第5期321-325,共5页Progress in Obstetrics and Gynecology

基　　金：国家985基金项目(No:985-2-015-24)

摘　　要：目的:了解母性效应基因Mater表达与人类卵母细胞及早期胚胎发育不同阶段的关系。方法:用巢式逆转录多聚酶链式反应(single cell nested RT-PCR)方法检测人类卵子和植入前各阶段胚胎的母性效应基因Mater mRNA的表达。结果:Mater在人类卵母细胞GV、MⅠ、MⅡ都有表达,植入前胚胎1、2、4、6细胞期表达量逐渐下降,到8细胞期、囊胚、孵出囊胚期均未检测到Mater mRNA表达。结论:人类卵母细胞和植入前胚胎Mater基因的表达量随发育时间的改变而变化,对卵子生长和胚胎发育有重要意义,与母性效应基因在小鼠等其他哺乳动物中的表达模式相似。Objective：To investigate expression of maternal effect gene Mater in human oocytes and preimplantation embryos and the effect of Mater gene expression on the development of human oocytes and embryos. Methods：The expression of maternal effect gene Mater in human oocytes and preimplantation embryos were tested by nested reverse transcriptionpoly- merase chain reaction（RT-PCR）. Results：Mater was expressed in human oocytes of different developmental stages. After fertilization, the mRNA expression slowly decreased in embryos during 1-cell to 6-cell stage;it was hardly detected in 8-cell stage, blastocysts and hatched blastocysts. Conclusion：The Mater expression decreases along with the stages of embryo development and may play an important role in human oocyte growth and preimplantation embryo develop- ment. Human Mater transcript expression patterns are consistent with the expression paterns in mouse or other mammalians.

关 键 词：母性效应基因 Mater 卵母细胞 胚胎 植入前 巢式RT-PCR 

分 类 号：R321.2[医药卫生—人体解剖和组织胚胎学]