雌激素膜受体GPR30在子宫内膜癌的表达及意义  

The expression and significance of estrogen transmembrane receptor GPR30 in endometrial carcinoma

作　　者：庄研[1] 张树泉[1] 贺银燕[1] 鲍伟[1] 杨懿霞[1] 万小平[1] 

机构地区：[1]上海交通大学附属第一人民医院妇产科,上海200080

出　　处：《现代妇产科进展》2009年第5期370-374,共5页Progress in Obstetrics and Gynecology

基　　金：上海市卫生局医学重大课题项目(No:2005ZD002);国家自然科学基金项目(No:30872760)

摘　　要：目的:探讨雌激素膜受体GPR30在子宫内膜癌的表达及意义。方法:RT-PCR和免疫组织化学法检测子宫内膜癌组织和正常子宫内膜组织中GPR30mRNA和蛋白的表达,分析GPR30表达与临床病理的联系;免疫细胞化学分析GPR30蛋白在子宫内膜癌细胞系RL95-2(ER+)和KLE(ER-)的表达。结果:子宫内膜癌组织GPR30mRNA的表达水平显著高于正常子宫内膜组织(P<0.05);子宫内膜癌组织GPR30蛋白表达阳性率(21/30,70%)显著高于正常子宫内膜组织(5/17,29.41%)(χ2=7.232,P=0.007);肿瘤组织学分级越高,GPR30表达阳性率越高(P=0.003),GPR30表达与内膜癌分期、肌层浸润深度和腹水转移无关(P>0.05);ERα阳性的子宫内膜癌细胞系RL95-2和ERα阴性的细胞系KLE均表达GPR30。结论:雌激素膜受体GPR30于子宫内膜癌组织和子宫内膜癌细胞系均有表达,与肿瘤组织学高分级相关,提示GPR30可能在子宫内膜癌中发挥重要的作用。Objective：To investigate the expression and significance of estrogen transmembrane receptor GPR30 in endometrial carcinoma. Methods： RT-PCR and immunohistochemistry （IHC） were used to assess the expression of GPR30 in the tissue of endometrial carcinoma and normal endometrium to evaluate the relationship between the expression of GPR30 and the clinicopathology. Immunoeytochemistry（ICC） was used to investigate the expression of GPR30 in endometrial cancer cell lines of RL95-2 （ER ＋ ） and KLE （ER - ）. Results： The transcriptional level of GPR30 was significantly higher in the tissue of endometrial carcinoma than that in normal endometrium （P 〈0.05）. The positive expression rate （21/30,70%） of GPR30 protein in endometrial carcinoma tissue was higher than that in normal endometrium tissue （5/17,29.41%） （ X^2 = 7. 232, P = 0. 007）. GPR30 expression was positively correlated with the grade of endometrial carcinoma. However, no correlation was found between the expression of GPR30 and the stage of the carcinoma, invasion, ascites metastasis （P 〉 0.05 ）. GPR30 expression was observed both in ER positive RL95-2 cell line and ER negative KLE cell line. Conclusion：Estrogen transembrane receptor GPR30 was expressed in endometrial carcinoma tissues as well as in endometrial cancer cell lines and the expression is positively related to the grade of the carcinoma, which suggested an important role of GPR30 in endometrial carcinoma.

关 键 词：子宫内膜肿瘤 GPR30 受体 雌激素 受体 雌激素膜 

分 类 号：R737.33[医药卫生—肿瘤]