何首乌及其混伪品序列相关扩增多态性反应体系的优化  

Optimization of SRAP-PCR in Polygonum multiflorum Thunb.and its Adulterants

作　　者：呙军[1] 赵树进[2] 郑传进[1] 

机构地区：[1]华南理工大学生物科学与生物工程学院,广东广州510640 [2]中国人民解放军广州军区广州总医院,广东广州510010

出　　处：《时珍国医国药》2009年第5期1137-1139,共3页Lishizhen Medicine and Materia Medica Research

基　　金：广州市科学技术基金资助项目(No.2007Z3-E5151)

摘　　要：目的建立何首乌及其混伪品序列相关扩增多态性(SRAP)分子鉴定技术体系。方法正交优化影响SRAP-PCR的Mg2+、dNTPs、引物浓度与Taq聚合酶量。结果20μl反应体系的优化组合为:Mg2+2.5 mmol/L,dNTPs 0.4 mmol/L,引物0.35μmol/L,Taq酶1U。结论Mg2+、dNTPs、引物浓度与Taq聚合酶量对不同物种SRAP-PCR结果有不同程度影响,利用SRAP分子鉴定前应对此4因素进行优化。Objective In order to characterize Polygonum multiflorum Thunb. and its adulterants, the SRAP molecular marker system was established. Methods Orthogonal design was used to optimize the concentrations of Mg^2＋ , dNTPs, primers and Taq DNA olymerase. Results The optimum system of 20 μl was as follows ： Mg^2＋ 2.5 mmol/L, dNTPs 0.4 mmol/L, primer 0.35 μmol/L, Taq DNA polymerase 1 U. Conclusion The concentrations of Mg^2＋ , dNTPs, primers, Taq DNA olymerase have different effect in the result of SRAP - PCR in different species. It is necessary to optimize these four factors before using SRAP to identity different species.

关 键 词：何首乌 混伪品 序列相关扩增多态性 优化 

分 类 号：R284.2[医药卫生—中药学]