大黄素对急性角膜炎症反应中ICAM-1表达的影响及意义  

Effect of emodin on expression of ICAM-1 in the corneas of acute keratitis

作　　者：陈国玲[1,2] 陈双 肖瑛[2] 毛淑芝[2] 高雪[2] 刘执玉[1] 

机构地区：[1]山东大学医学院解剖学教研室,济南250012 [2]山东大学眼科中心,第二医院眼科,济南250033 [3]枣庄市山亭区人民医院,山东枣庄277200

出　　处：《山东大学学报（医学版）》2009年第5期31-33,37,共4页Journal of Shandong University:Health Sciences

基　　金：山东省教育厅科技计划项目(J08LH59)

摘　　要：目的观察大黄素对急性角膜炎大鼠角膜组织中细胞间粘附分子-1(ICAM-1)表达的影响及意义。方法建立角膜炎大鼠模型,模型制备前30 min,大鼠结膜下分别注射大黄素(治疗组)及等容二甲基亚砜(炎症组)。按脂多糖(LPS)刺激后不同时点,各组又分为1、3、6、122、4 h组,每亚组8只鼠。于各时点观察大鼠的全身情况,裂隙灯显微镜观察大鼠结膜、角膜及房水等眼部表现;抽取大鼠房水涂片,HE染色观察房水细胞的变化;角膜组织切片,HE染色观察各组大鼠角膜的组织形态学改变;逆转录-聚合酶链反应(RT-PCR)检测大鼠角膜组织中ICAM-1 mRNA的表达。结果球结膜下预防性注射大黄素,可明显改善角膜炎大鼠畏光、流泪等眼部表现,减轻角膜混浊、组织水肿等炎症反应。大黄素预处理可明显减轻大鼠角膜组织及房水中炎症细胞浸润的程度,改善角膜组织水肿及角膜基质层胶原纤维排列的紊乱程度。LPS刺激可引起炎症组大鼠角膜组织中ICAM-1mRNA表达增加,该作用可被大黄素部分抑制,治疗组各时点ICAM-1 mRNA的表达较炎症组均明显降低(P均<0.01)。结论大黄素可减少急性角膜炎症反应中ICAM-1 mRNA的表达,对急性角膜炎大鼠角膜损伤起保护作用。Objective To investigate the signification and effect of emodin on expression of intercellular adhesion molecule-1 （ICAM-1） in the corneas of acute keratitis-induced by lipopolysaccharide （LPS） in Wistar rats. Methods Pseudomonas aeruginosa LPS-induced keratitis models were established in Wistar rats. Thirty minutes before LPS exposure, emodin and its vehicle were separately injected into sub-conjunctival tissues in Wistar rats. At 1, 3, 6, 12 and 24 hours after being challenged with LoS, signs of both groups were observed, and severity of rats＇ ocular inflammation was examined with a slit lamp microscope. The aqueous humor of each group was extracted and examined with hematoxytin and eosin（HE）-stained, and expression of ICAM- 1 mRAN was detected by reverse transcriptase-polymerase chain reaction（RT-PCR）. Results Ocular behavior was improved in rats which had undergone sub-conjtmctival injection with emodin before being challenged with LPS. Inflammatory cells of the cornea and aqueous humor were less in rats of the treatment group than those of the inflammation group. The corneal edema and collagen fibers which appeared in disarrangement were also improved after emodin treatment. Expression of ICAM-1 mRNA of the treatment group was increased in each point after being challenged with LPS（ P 〈 0.01）, which was significantly inhibited by pretreatment with emodin（ P 〈 0. 01）. Conclusion Emodin can decrease expression of ICAM-lmRNA induced by LPS in the corneas of rats, and protects against acute corneal injury and improves rats＇ symptoms.

关 键 词：大黄素 炎症 角膜 大鼠 Wistr 

分 类 号：R772.2[医药卫生—眼科]