p-STAT3、MMP-2及VEGF在视网膜母细胞瘤中的表达及意义  

Expressions of p-STAT3,MMP-2 and VEGF in retinoblastoma

作　　者：杨乐[1] 申家泉[1] 程育宏[1] 

机构地区：[1]山东大学附属省立医院眼科,济南250021

出　　处：《山东大学学报（医学版）》2009年第5期113-116,共4页Journal of Shandong University:Health Sciences

基　　金：山东省自然科学基金资助项目(Y2002C32)

摘　　要：目的探讨信号传导和转录激活因子3(STAT3),基质金属蛋白酶-2(MMP-2)和血管内皮生长因子(VEGF)在视网膜母细胞瘤(RB)中的表达及其与临床病程的关系。方法用免疫组织化学SP法分别检测39例RB中p-stat3、MMP-2及VEGF的表达;用全自动医学图像分析系统(LEICA IM,50型)测量其平均灰度值,比较不同临床和病理阶段RB中表达p-STAT3、MMP-2和VEGF的差异及三者阳性表达之间的关系。结果39例RB标本中p-STAT3阳性表达26例,占66.7%(26/39);MMP-2阳性表达31例,占79.5%(31/39);VEGF阳性表达25例,占64.1%(25/39);p-STAT3、MMP-2和VEGF表达水平均与RB视神经浸润呈正相关(P<0.05、P<0.01、P<0.01)与病理分化程度呈负相关(P<0.01、P<0.01、P<0.05),而与患者年龄、性别以及眼别差异均无统计学意义(P>0.05);p-STAT3和MMP-2表达水平呈密切相关(P<0.01),p-STAT3与VEGF表达水平成密切相关(P<0.01)。结论p-STAT3、MMP-2、VEGF的表达水平可能与RB的浸润转移有关,三者共同促进RB的浸润转移。Objective To investigate the expression of signal transduction and activators of transcription-3（p-STAT3）, matrix metalloproteinase-2 （MMP-2） and vascular endothelial growth factor（VEGF ）in retinoblastoma（ RB ） and the relationships among p-STAT3, MMP-2 VEGF and tumor development. Methods Immunohistochemical technique was used to detect expressions of p-STA33, MMP-2 and VEGF in 39 cases of paraffin embedded RB samples. Quantitative analysis of expressions of p-STAT3, MMP-2 and VEGF were assessed by measuring the mean gray scale of RB tissues with LEICA IM 50 Color Pathologic Analysis System. Differences of expressions of p-STAT3, MMP-2 and VEGF in each clinical and pathological stage were statistically analyzed. Results The positive expression rate of p-STAT3 was 66.7%（26/39）, of MMP-2 was 79.5 %（31/39）and of VEGF was 64.1% （25/39）. There was a statistically significant positive correlation between expressions of p-STA33, MP2 and VEGF with optic nerve invasion（ P 〈 0.05, P 〈 0.01, P 〈 0.01 ）, also there was a statistically significant negative correlation between expressions of p-STAT3, MMP2 and VEGF with differentiation of cancers（ P 〈 0.01, P 〈 0.01, P 〈 0.05）, but there was no correlation with age, gender and diseased eyes of patients with RB. Also, there was a statistically significant positive correlation between expression of p-STAT3 and expression of MMP-2 （ P 〈 0.01 ） and between expression of p-STA73 and expression of VEGF （ P 〈 0.01）. Conclusion The positive expression levels of p-STATS, MMP-2 and VEGF may correlate with tumor invasion and metastasis and they may promote invasion and metastasis of RB.

关 键 词：视网膜母细胞瘤 信号传导和转录激活因子3 基质金属蛋白酶-2 血管内皮生长因子 免疫组织化学 

分 类 号：R774.1[医药卫生—眼科]