REM睡眠剥夺对大鼠额叶皮质5-TH的含量及学习记忆的影响  

Effects of REM sleep deprivation on cognitive function and content of 5-HT in rat frontal cortex

作　　者：李训军[1] 严家川[1] 潘峰[1] 蒋晓江[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所神经内三科,重庆400042

出　　处：《重庆医学》2009年第10期1151-1152,1154,共3页Chongqing medicine

摘　　要：目的观察不同持续时间快速眼动相(REM)睡眠剥夺对大鼠额叶皮质内5-羟色胺(5-TH)的含量及学习记忆的影响。方法采用改良多平台水环境法建立大鼠REM睡眠剥夺模型,高效液相色谱电化学法检测大鼠额叶皮质内5-TH的含量,Morris水迷宫检测大鼠睡眠剥夺前后的学习记忆变化。结果与正常对照组相比,随着REM睡眠剥夺的延长,大鼠额叶皮质内5-TH的含量增加,同时大鼠的学习记忆能力较前下降。结论REM睡眠剥夺导致大鼠学习记忆能力下降,可能与额叶皮质内5-TH含量增加有关。Objective To investigate the effects of rapid eye movement （REM） sleep deprivation on cognitive function and the content of 5-HT in the rat frontal cortex. Methods Experimental model of sleep deprivation （SD） was established by use of flow pot method. High performance liquid chromatography-electrochemical detection（HPLC-ECD） technique was used to analyze the levelsof 5-HT. The variation of learning and memory was detected by Morris water maze at pre and post sleep deprivation. Results As compared with control group, the content of 5-HT in frontal cortex increasing accompanied with the REM sleep deprivation time prolonging,while the learning ability was decreasing. Conclusion The dysfunction of learning ability of sleep deprivation may be associated with the content of 5-HT in the rat frontal cortex.

关 键 词：快速眼动相睡眠剥夺 学习记忆 MORRIS水迷宫 5-羟色胺 

分 类 号：R363[医药卫生—病理学]