3′-甲氧基葛根素抗大鼠脑缺血再灌注损伤作用  

The protective role of 3′-Methoxypuerarin in hippocampal neuronsagainst ischemia/reperfusion injury in rats

作　　者：刘亚君[1] 陈筱南[2] 刘克敬[1] 柴强[3] 李景新[1] 孙东 陈连璧[1] 

机构地区：[1]山东大学医学院,济南250012 [2]山东省医学科学院附院 [3]山东省医学科学院生理研究所 [4]济南市食品药品监督管理局历下分局

出　　处：《山东医药》2009年第20期13-15,共3页Shandong Medical Journal

基　　金：山东省自然科学基金资助项目(Y2005C69)。

摘　　要：目的探讨3′-甲氧基葛根素(3-′MOP)在大鼠脑缺血再灌损伤中的神经保护作用。方法24只大鼠随机分为脑缺血再灌组(IR组)、IR+3-′MOP组和对照组。HE染色观察海马CA1区神经元的组织形态学变化;TUNEL染色检测海马CA1区神经元阳性凋亡细胞数。结果IR+3-′MOP组海马CA1区神经元的损伤减轻,细胞存活数显著多于IR组,而凋亡细胞数显著少于IR组。结论3-′MOP可经抑制凋亡的发生,减轻海马CA1区神经元缺血再灌损伤。Objective To investigate the protective role of 3＇-Methoxypuerarin （3＇-MOP） in hippocampal neurons against ischemia/reperfusion injury in rats. Method Rats were randomly divided into an ischemia/reperfusion （IR） group, an IR ＋ 3＇-MOP group and a control group. Histological changes in the neurons of the hippocampal CA1 region were observed with HE staining. The apoptotic neurons in hippocampal CA1 area were counted with TUNEL staining. Result Compared with IR group, 3＇-MOP could increase the number of surviving neurons in hippocampal CA1 region（ P 〈 0.01 ） and markedly reduce the number of apoptotic pyramidal neurons（P 〈 0.01 ）after ischemia/repetfusion injury. Conclusion 3 ＇-Methoxypuerarin can protect the hippocampal neurons against ischemia/reperfusion injury via reducing the inhibiting apoptosis.

关 键 词：3'-甲氧基葛根素 海马神经元 脑缺血再灌损伤 凋亡 大鼠 

分 类 号：R743.3[医药卫生—神经病学与精神病学]