JC病毒大T抗原在人结直肠腺癌中的表达及其与P53、pRb表达的关系  

Expression of JC Virus Large Tumor Antigen in Human Colorectal Adenocarcinoma and Its Relationship with the P53,pRb Proteins

作　　者：孟轶婷[1] 万义增[1] 赵丽娟[2] 杨京京[1] 何丽馥[1] 李敬岩[1] 

机构地区：[1]辽宁医学院附属第三医院病理科,辽宁锦州121001 [2]辽宁医学院附属第三医院消化内科,辽宁锦州121001

出　　处：《癌变．畸变．突变》2009年第3期226-229,共4页Carcinogenesis,Teratogenesis & Mutagenesis

摘　　要：背景与目的:探讨JC病毒(JCV)早期基因编码产物大T抗原(1arge tumor antigen,T-Ag)的表达及与抑癌蛋白P53、pRb的关系及在人结直肠腺癌发生发展中的意义。材料与方法:应用免疫组织化学PV二步法检测77例结直肠腺癌,20例结直肠腺瘤和20例正常肠粘膜中JCV T-Ag、P53、pRb的表达情况。结果:77例结直肠腺癌组织中JCV T-Ag、P53和pRb表达阳性率分别为36.4%、61.0%和55.8%,与腺瘤组织和正常肠粘膜比较差异均具有统计学意义(P均<0.05)。JCV T-Ag在结直肠腺癌的表达与组织学分级和临床病理分期无关(P>0.05),P53与组织学分级相关(P<0.05),pRb与TNM分期、淋巴结转移有关(P<0.05)。JCV T_Ag与P53、pRb表达之间存在明显相关性(r=0.272,r=0.237,P均<0.05)。结论:JCV与人类结直肠腺癌发生密切相关,其编码产物大T抗原在致癌过程中起重要作用。BACKGROUND AND AIM： To explore the role of JC virus early region gene encoding product large tumor antigen（T-Ag） and the interaction between T-Ag and tumor suppressors P53 and pRb in human eolorectal adenoearcinoma. MATERIALS AND METHODS： The expression of JCV T-Ag and P53 and pRb proteins were evaluated by immunohistoehemistry PV method in 77 cases of human colorectal adenocareinoma , 20 coloreetal adenomas and 20 normal colorectal mueosa. RESULTS： The positive rate of JCV T-Ag , P53 and pRb proteins were 36.4%, 61.0%and 55.8%, respectively, in 77 cases of human colorectal adenocareinoma , and there was an obvious difference when compared with colorectal adenomas and normal colorectal mucosa （P 〈 0.05）.There was no statistically significant links between the presence of T-Ag and pT, pN, pM or differentiation degree（P〈0.05） .P53 was closely correlated to differentiation degree（P 〈 0.05）,but was not correlated to the age, site, p-TNM and lymph node metastasis（P 〉 0.05）. pRb was closely correlated to p-TNM and lymph node metastasis（ P 〈 0.05）.There was statistically significant correlation among the expression of TAg and P53, pRb protein （r = 0.272, r = 0.237, P 〈 0.05） . CONCLUSION： JCV was closely related to colorectal adenocarcinoma. Its encoding product in the large T antigen played an important role in the carcinogenic process.

关 键 词：JC病毒 大T抗原 P53 PRB 结直肠腺癌 

分 类 号：R735.3[医药卫生—肿瘤]