东北刺人参体细胞胚状体的诱导及体细胞胚胎发生的扫描电镜观察  

Induction and Observation of the Somatic Embryogenesis of Oplopanax elatus by Scanning Electron Microscope

作　　者：夏广清[1] 朱俊义[1] 王艳萍[1] 

机构地区：[1]通化师范学院生物系,通化134001

出　　处：《吉林农业大学学报》2009年第2期174-177,共4页Journal of Jilin Agricultural University

基　　金：吉林省教育厅科研项目[2006(151)]

摘　　要：分别以东北刺人参叶芽和带有叶柄的幼叶为外植体,研究6-BA浓度对东北刺人参体细胞胚状体诱导的影响,并利用扫描电镜对体细胞胚胎发生的形态学进行了观察。结果表明:以带有叶柄的东北刺人参幼叶为外植体,在MS培养基上附加4.0 mg/L 6-BA和0.5 mg/L NAA,可获得健状的胚状体,并且胚状体经过球形胚、心形胚、鱼雷形胚和子叶形胚的胚胎发育时期,最终形成可发育成完整小植株的成熟胚。The leaves with leafstalk and buds of Oplopanax elatus Nakai were taken as explants and cul- tured in vitro. Large and healthy embryoids were obtained on MS medium supplement with 4.0 mg/L, 0.5 mg/L NAA from buds. The development of somatic embryos of Oplopanax elatus Nakai was observed by scanning electron microscopy. The results showed that the embryoids were developed from global, heart-shaped, torpedo-shaped and cotyledon embryo, then matured and formed planet.

关 键 词：东北刺人参 体细胞胚状体 诱导 扫描电镜 

分 类 号：S567.51[农业科学—中草药栽培]