Annexin Ⅱ在肺鳞癌、腺癌中的表达及意义  

The Expression and Significance of Annexin Ⅱ in Lung Squamous Cell Cancer and Adenocarcinom

作　　者：许月新[1,2] 刘东明[1,3] 段鸿梅[1] 官忠艳[1] 韩昱晨[1] 

机构地区：[1]中国医科大学基础医学院病理学教研室,沈阳110001 [2]北京市门头沟区医院病理科,北京102300 [3]内蒙古民族大学附属医院内分泌科,通辽028000

出　　处：《中国肺癌杂志》2009年第5期375-380,共6页Chinese Journal of Lung Cancer

基　　金：辽宁省自然基金(No.20052085);辽宁省教育厅基金(No.20060958)资助~~

摘　　要：背景与目的AnnexinⅡ是一类钙依赖性磷脂结合蛋白,在许多的细胞功能中发挥作用,已有研究显示annexinⅡ参与肿瘤的发生发展。本研究通过检测膜联蛋白Ⅱ(annexinⅡ)在肺癌组织中的表达情况,分析annexinⅡ与各临床病理因素间的关系。方法应用免疫组组织化学(SP法)检测110例肺鳞癌和肺腺癌组织中annexinⅡ的表达;应用WesternBlot方法检测40例肺鳞癌和肺腺癌组织及相应癌旁正常肺组织中annexinⅡ蛋白的表达。结果在110例肺癌组织中,有67例为annexinⅡ高表达(60.9%),鳞癌为胞膜表达,腺癌为胞膜表达或胞膜与胞浆均有表达,但以胞膜表达为主。AnnexinⅡ的表达情况与肺癌患者的淋巴结转移(P=0.008)及TNM分期(P=0.002)相关。AnnexinⅡ蛋白在肺鳞癌、腺癌和癌旁组织中均有表达,在癌组织中的表达明显高于癌旁正常肺组织(P<0.001)。结论AnnexinⅡ蛋白的表达与肺鳞癌及腺癌的发展密切相关。Background and objective Annexin Ⅱ is a calcium-dependent phosphnolipid-binding protein that plays a role in many cellular fimetions. It has been proven that annexin Ⅱ has been involved in many human cancers. The aim of this study is to examine the expression of annexin Ⅱ in lung cancer tissues, and analyze the correlations between annexin Ⅱ and elinicopathological parameters. Methods Annexin Ⅱ expression was examined in 110 lung cancer specimens using immunohistoehemistty （SP method）. Western Blot was used to detect the expression of annexin Ⅱ in hmg cancer tissues and the corresponding normal lung tissues. Results Among 110 lung cancer specimens, 67 samples showed positive expression of annexin Ⅱ （60.9%）. It showed membranous staining in lung squamous cell carcinoma. The positive membranous expression and cytoplasmic expression of annexin Ⅱ in lung adenoeareinoma mainly showed membranous staining. Annexin Ⅱ expression level was positively correlated with lymphoid node metastasis （P=0.008） and TNM stages （P=0.002）. There was expression of annexin Ⅱ in both lung cancer tissues and the corresponding normal lung tissues, and in lung cancer tissues, the total levels of annexin Ⅱ proteins were significantly higher than that in the corresponding normal lung tissues （P〈0.001）. Conclusion The expression of annexin Ⅱ positively may correlated with lung squamous cell carcinoma and adenoeareinoma development.

关 键 词：膜联蛋白Ⅱ 肺肿瘤 免疫组织化学 蛋白印记杂交 

分 类 号：R734.2[医药卫生—肿瘤]