雷公藤氯内酯醇对大鼠海马注射β淀粉样蛋白后诱导型一氧化氮合酶的影响  

Effects of Tripchlorolide on iNOS Induced by β-amyloid in Rat Hippocampus

作　　者：陈龙飞[1] 许国英[1] 吴钢[1] 

机构地区：[1]福建医科大学附属第一医院神经内科,350005

出　　处：《中国临床神经科学》2009年第3期231-234,共4页Chinese Journal of Clinical Neurosciences

基　　金：福建省中医药重点课题(编号:wzz10602)

摘　　要：目的:研究雷公藤氯内酯醇(T4)对阿尔茨海默病(AD)大鼠脑内诱导型一氧化氮合酶(iNOS)表达及NO产生的影响。方法:通过大鼠海马注射Aβ1-40建立AD大鼠模型,以腹膜腔注射T4作为干预措施,免疫组化方法检测iNOS,硝酸还原酶法检测硝酸盐/亚硝酸盐水平。结果:Aβ组大鼠海马iNOS表达和NO产生较假手术组明显增加,Aβ+T4组大鼠海马iNOS表达和NO产生较Aβ组减少。结论:T4可以抑制Aβ1-40诱导的iNOS表达和NO产生。Aim： To explore the effects of tripchlorolide on the expression of iNOS protein and the production of NO in brains of Alzheimer＇ s disease rats. Methods： Alzheimer＇s disease model was established by injecting Aβ1-40 into rat hippocampus. Tripchlorolide was injected into abdominal cavity as the treatment method. The expression of iNOS protein was assessed by using immunohistochemical method, the NO level was assessed by nitrate reductase method. Results： The levels of iNOS and the NOx expressions were increased in the hippocampus of Aβ group compared with the sham operated group. The levels of iNOS and the NOx expressions were decreased in hippocampus of tripchlorolide group compared with Aβ group. Conclusion： Tripchlorolide could inhibite the expression of iNOS protein and the production of NO induced by Aβ1-40.

关 键 词：雷公藤氯内酯醇 阿尔茨海默病 大鼠 β淀粉蛋白 诱导型一氧化氮合酶 

分 类 号：R749.1[医药卫生—神经病学与精神病学]