重组hDCN对肝癌细胞株HepG2表面HLA分子表达的影响  

Effect of rhDCN on the expression of HLA molecules on HepG2 cell lines

作　　者：王艳红[1] 王桂琴[1] 王晶晶[1] 郝芳[1] 杨婉芳[1] 薛莹[1] 

机构地区：[1]山西医科大学微生物学与免疫学教研室,太原030001

出　　处：《肿瘤研究与临床》2009年第5期298-300,共3页Cancer Research and Clinic

基　　金：山西医科大学大学生创新基金;山西省自然科学基金（2007011107）

摘　　要：目的研究peDNA3．1（＋）-核心蛋白聚糖（DCN）重组质粒对体外培养的HepG2细胞株表面HLAⅠ、Ⅱ类分子表达的影响，探讨DCN增强抗肿瘤免疫应答的作用。方法重组质粒pcDNA3．1（＋）-DCN转染HepG2细胞，用流式细胞术（FCM）检测转染前后HepG2细胞表面HLAⅠ、Ⅱ类分子的表达。结果HepG2细胞低表达HLAⅠ、Ⅱ类分子，经转染重组质粒作用后，HLAⅠ、Ⅱ类分子的荧光强度明显增强。结论rhDCN核心蛋白能上调肿瘤细胞表面HLAⅠ、Ⅱ类分子的表达，这一作用可能参与其抗肿瘤效应机制。Objective To observe the regulatory effect of recombinant human DCN on the expression of HLA class Ⅰ , Ⅱ molecule on hepatoma carcinoma cell HepG2 and investigate the relatively immune mechanism of human DCN on enhancing anti-tumor effect. Methods After transfected HepG2 cells with pcDNA3.1（＋）-DCN by liposome transfection, the expression of HLA class Ⅰ , Ⅱ molecule and the apoptotic indexes were analyzed by the flow cytometer. Results The expression of HLA class Ⅰ , Ⅲ molecule on the HepG2 cells transfected with pcDNA3.1 （＋）-DCN was upregulated with the apoptosis indexes being significantly higher than that of other control groups. Conclusion The human DCN can induce HepG2 apoptosis and up-regulate the expressions of HLA class Ⅰ , Ⅱ molecule which may contribute to its antitumor effect.

关 键 词：蛋白聚糖类 HEPG2 基因 MHC Ⅰ类 基因 MHCⅡ类 

分 类 号：R735.7[医药卫生—肿瘤]