子宫颈癌中Gli蛋白过表达的临床病理学意义  

Clinicopathological significance of Gli proteins overexpression in uterine cervical cancers

作　　者：玄延花[1] 李贵铃[1] 金铁峰[1] 林贞花[1,2] 

机构地区：[1]延边大学医学院病理学教研部,延吉133000 [2]延边大学长白山生物功能分子教育部重点实验室,延吉133002

出　　处：《临床与实验病理学杂志》2009年第2期122-126,共5页Chinese Journal of Clinical and Experimental Pathology

基　　金：国家教育部重点项目"新世纪优秀人才支持计划"(NCET-07-0734);国家自然科学基金(30560047);国家教育部留学回国人员科研启动基金(教外司留20071108);吉林省科学发展计划杰出青年基金(20060103)

摘　　要：目的研究Gli蛋白检测在子宫颈癌及其癌前病变中的临床病理学意义,并分析Gli蛋白表达与HPV16型感染的关系。方法32例正常子宫颈上皮、71例子宫颈上皮内瘤变(CIN);其中CIN128例、CIN218例、CIN325例和80例子宫颈鳞状上皮癌(SCC)共183例存档蜡块选自延边大学医院、延边妇幼保健院和延边肿瘤医院病理科。应用PCR技术检测上述组织中HPV16型的感染,并用免疫组化方法在子宫颈病变组织芯片中检测Gli-1、Gli-2、Gli-3蛋白的表达情况。结果Gli-1和Gli-2蛋白呈胞质和胞核染色,Gli-3蛋白多数呈胞质染色。在子宫颈癌和癌前病变(CIN2/3)中,Gli-1～3呈强阳性,其阳性表达率均显著高于正常子宫颈黏膜上皮。80例子宫颈癌标本中HPV16阳性率是77.5%。在子宫颈癌组织中HPV16阳性组的Gli-1蛋白的胞质阳性率(85.5%,53/62)与强阳性率(85.5%,53/62)以及Gli-2蛋白的胞质阳性率(100%)与胞核强阳性率(100%)均高于HPV16阴性组(Gli-1胞质阳性率与强阳性率均为0,Gli-2胞质阳性率和胞核强阳性率分别为0和50%),差异具有统计学意义(P均<0.05);而3种蛋白表达率与患者的年龄、淋巴结转移、肿瘤学分期和临床分期均无关。结论Gli-1、Gli-2、Gli-3蛋白过表达可以作为子宫颈癌及其癌前病变的早期辅助诊断指标,Gli蛋白的表达水平与HPV16感染密切相关,且有望成为子宫颈癌靶向治疗的新靶点。Purpose To investigate the significance of Gli proteins expression in uterine cervical lesions and its relationship with HPV 16 infection status. Methods Total 183 cases of cervical lesions, including 32 non-neoplastic cervix, 71 cervical intraepithelial neoplasia （28 CIN1, 18 CIN2,and 25 CIN3） and 80 squamons cell carcinomas （SCCs） were selected from the Department of Pathology, Yanbian University Hospital, Yanbian Women Hospital, and Yanbian Tumor Hospital. Gli-1 - 3 proteins expression was detected in tissue mieroarray by immunohistochemistry,and HPV 16 screening was performed by using PCR method. Results Gli-1 and Gli-2 were both cytoplasmic and nuclear staining pattern, but Gli-3 was mainly observed in the cytoplasm. The immunohistochemical staining showed that the expression rates of Gli-1, 2 and Gli-3 were significantly higher in cervical cancer and precursor lesions （ CIN2/3 ） than normal cervical epithelia. Meanwhile,HPV16 was positive in 77.5% of SCCs. In cervical cancer tissues, the expression rates of Gli-1 （both positive and strong positive rates of cytoplasmic pattern were 8.5.5% ） and Gli-2 （positive rate of cytoplasmic pattern was 100% ; strong positive rate of nuclear pattern was 100% ） were significantly higher in HPV16 positive group than in HPV16 negative group （ both positive and strong positive rates of Gli-1 cytoplasmic pattern were 0 ; positive rate of Gli2 cytoplasmic pattern was 0 ; strong positive rate of Gli-2 nuclear pattern was 50% ） （ P 〈 0. 05 ）. Conclusions Gli-1 - 3 proteins of hedgehog signaling molecules might be a helpful marker for the early diagnosis and a potential therapeutic target for cervical cancers. Gli protein expression level is also related to the HPV16 infection status.

关 键 词：子宫肿瘤 Gli蛋白 乳头状瘤病毒 人 

分 类 号：R737.33[医药卫生—肿瘤]