甲状腺肿瘤中survivin和Ki-67的表达  

Expression of survivin and Ki-67 and their significance in thyroid tumors

作　　者：金东岭[1] 李兰梅[1] 刘现军[1] 梁爽[1] 席丰[1] 高金生[1] 

机构地区：[1]河北工程大学医学院病理教研室,邯郸056002

出　　处：《临床与实验病理学杂志》2009年第2期154-157,共4页Chinese Journal of Clinical and Experimental Pathology

基　　金：河北省科技攻关项目(06276102D-55)

摘　　要：目的探讨凋亡抑制蛋白survivin和Ki-67抗原在甲状腺肿瘤组织中的表达及其临床病理意义。方法选择术后切除的甲状腺癌标本56例、甲状腺腺瘤13例、腺瘤旁正常甲状腺组织10例,采用SP法染色,观察survivin、Ki-67抗原的表达情况,结合临床病理资料进行统计学分析。结果正常甲状腺组织、甲状腺腺瘤和甲状腺癌患者中survivin阳性表达依次为0、15.38%和60.71%;Ki-67阳性表达依次为0、23.08%和69.64%,甲状腺癌中survivin和Ki-67的表达明显高于正常甲状腺组织和甲状腺腺瘤(P<0.05)。年龄>45岁组甲状腺癌survivin表达明显高于<45岁组(P<0.05);而Ki-67表达无年龄差异(P>0.05)。survivin和Ki-67表达与甲状腺癌病理类型无关(P>0.05)。TNM分期Ⅲ、Ⅳ期患者的survivin和Ki-67阳性表达明显高于Ⅰ、Ⅱ期患者(P<0.05)。在有淋巴结转移的甲状腺癌中survivin和Ki-67阳性表达明显高于无淋巴结转移者(P<0.05)。结论survivin的高表达与甲状腺癌的发生、发展和转移有密切关系。survivin和Ki-67的表达情况对甲状腺癌的诊断和预后评估有一定临床意义。二者联合检测对甲状腺癌的早期诊断可能发挥重要作用。Purpose To study the expression of survivin and Ki-67 protein in thyroid tumor and the relation with parameters of clinical pathology. Methods The expression of survivin and Ki-67 was examined and compared by immunohistoehemistry in 56 cases of thyroid carcinoma, 13 cases of thyroid benign tumor, and 10 cases of normal thyroid. The results were subjected to statistics analysis, combined with the clinical pathologic data. Results In normal thyroid, thyroid adenoma and thyroid carcinoma, the positive expression rate of survivin was 0,15.38% and 60. 71%, and that of Ki-67 was 0,23.08%, and 69. 64%, respectively. The expression of survivin and Ki- 67 in thyroid carcinoma was higher than that in normal thyroid and thyroid adenoma （ P 〈 0. 05 ）. The positive expression of survivin in those who were greater than 45-year-old was higher than those who were younger than 45-year-old （ P 〈 0. 05 ） ; but Ki-67 had no difference among the cases （ P 〉 0. 05 ）. The positive expression of survivin and Ki-67 had no significant difference in different pathologic types （P 〉 0.05 ）. The positive expression of survivin and Ki-67 in thyroid carcinoma were much higher than that in normal thyroid and thyroid adenoma （ P 〈 0. 05 ）. The positive expression of survivin in stages Ⅲ and Ⅳwas higher than that in stages Ⅰ and Ⅱ（ P 〈 0. 05 ）. The positive expression of survivin and Ki-67 in cases with lymph node metastasis was higher than that without lymph node metastasis. Conclusions The overexpression of survivin is involved in the metastasis and progression of thyroid carcinoma. The expression of survivin and Ki-67 may play a role in the diagnosis and judgment of malignancy of thyroid carcinoma. Combined detection of survivin and Ki-67 may help in the early diagnosis of thyroid carcinoma.

关 键 词：甲状腺肿瘤 SURVIVIN KI-67 免疫组织化学 

分 类 号：R736.15[医药卫生—肿瘤]