人白血病-NOD／SCID小鼠髓外浸润模型的建立与鉴定  

Establishment of a model of outer marrow infiltration of leukemia-NOD/SCID mice

作　　者：杨文华[1] 于文俊[2] 史哲新[1] 杨向东[1] 高宏[1] 汤毅[1] 吕俊秀[2] 

机构地区：[1]天津中医药大学第一附属医院血液科,300193 [2]天津中医药大学,300193

出　　处：《临床与实验病理学杂志》2009年第2期178-182,共5页Chinese Journal of Clinical and Experimental Pathology

基　　金：国家自然科学基金项目(30672743);天津市应用基础研究项目(07JCYBJC11500)

摘　　要：目的通过给NOD/SCID小鼠尾静脉注射白血病人骨髓单个核细胞(BMMNC)或CEM细胞系的方式,建立人白血病-NOD/SCID小鼠髓外浸润模型,并进行模型鉴定。方法将36只NOD/SCID小鼠经137Cs全身照射270cGy后,随机分为4组,每组均9只,在24h内尾静脉注射的方式接种白血病人BMMNC或CEM细胞系。(1)实验Ⅰ组:BMMNC1×107/只;(2)实验Ⅱ组:BMMNC2×107/只;(3)实验Ⅲ组:CEM细胞1×107/只;(4)对照组:生理盐水0.2ml/只。持续观察其一般情况和生存时间,监测外周血白细胞计数和涂片,并应用组织病理、流式细胞术等监测白血病细胞髓外浸润表现。结果注射4～8周后,3组实验组均发生髓外浸润,成功建立人白血病-NOD/SCID小鼠髓外浸润模型。实验Ⅰ组有5只成功建立模型;实验Ⅱ组有7只均成功建立模型;实验Ⅲ组9只均成功建立模型。结论应用尾静脉注射白血病人BMMNC或CEM细胞系的方法,均可成功建立人白血病-NOD/SCID小鼠髓外浸润模型,且尾静脉注射白血病人BMMNC成模率高,生存时间长,为白血病髓外浸润机制的研究和抗白血病髓外浸润药物的筛选提供了良好的实验模型。Purpose To establish a model of outer marrow infiltration of leukemia-NOD/SCID mice by injecting leukemia patient＇ s bone marrow mononuclear cells （BMMNC） and CEM cell line into tail vein. Methods Mice, irradiated 270 cGy on body by ^137 Cs, were randomly divided into four groups ,with each group of 9. Leukemia patient＇s bone marrow mononuclear cells（ BMMNC ）and CEM cell line were inoculated within 24 hours. （1）group Ⅰ ： BMMNC 1×10^7 per mouse; （2） group Ⅱ ： BMMNC 2 ×10^7 per mouse;（3 ） group m ： CEM cell 1 ×10^7per mouse; （4） control group：0. 2 ml saline per mouse. The general situation and the survival time were observed, and peripheral blood white blood cell count and smear, and outer marrow infiltration of leukemia ceils were examined by histopathology and flow cytometry. Results 4 - 8 weeks after the injection, all mice in the experiment groups had outer marrow infiltration, succeeded in setting up a model. The model was successfully established in Group Ⅰ （7 mice） , Group Ⅱ （7） and Group Ⅲ （9）. Conclusions The model of outer marrow infiltration of leukemia-NOD/SCID mice can be made by injecting leukemia patient＇ s bone marrow mononuclear cells（BMMNC） and CEM cell line into tail vein of mice, and this method has higher success rate of model establishment and longer survive time, providing a good model for study of mechanism of leukemia outer marrow infiltration and screening of the drugs of anti-leukemia outer marrow infiltration.

关 键 词：白血病 NOD/SCID 髓外浸润 疾病模型 动物 

分 类 号：R733.7[医药卫生—肿瘤] R363-332[医药卫生—临床医学]