血红素加氧酶介导GA对小麦糊粉层中α-淀粉酶的诱导表达  

Involvement of Heme Oxygenase in Gibberellic Acid-induced α-Amylase Expression in Wheat Aleurone Layer

作　　者：武明珠[1] 徐晟[1] 崔为体[1] 张博[1] 刘雅辉[1] 凌腾芳[1] 沈文飚[1] 

机构地区：[1]南京农业大学生命科学学院,南京农业大学-贝克曼离心机合作示范实验室,南京210095

出　　处：《植物生理学通讯》2009年第5期483-486,共4页Plant Physiology Communications

摘　　要：单独以赤霉素(GA)处理或与HO-1诱导物高铁血红素(Ht)和CO水溶液组合处理均导致小麦糊粉层中血红素加氧酶(HO)活性的提高,同时α-淀粉酶基因表达和α-淀粉酶活性也明显受诱导;用HO-1专一性抑制剂锌原卟啉(ZnPPIX)预处理6h后,上述效应部分受阻断。这暗示HO可能参与GA诱导的α-淀粉酶基因表达。In this study, we showed that gibberellic acid （GA） with or without HO-1 inducer hematin （Ht） or carbon monoxide （CO） aqueous solution not only led to promote heme oxygenase （HO） activity in wheat aleurone layer, but also enhanced α-amylase gene expression and its enzyme activities. Whereas, application of HO-1 inhibitor zinc protoporphyrin IX （ZnPPIX） for 6 h blocks partially above processes. Together, above results suggested that HO might be involved in GA-induced α-amylase gene expression.

关 键 词：小麦糊粉层 血红素加氧酶 高铁血红素 一氧化碳 赤霉素 Α-淀粉酶 

分 类 号：Q945[生物学—植物学] S512.1[农业科学—作物学]