他莫昔芬对SHG-44人胶质瘤细胞钠通道的抑制作用  

THE INHIBITION OF TAMOXIFEN ON SODIUM CHANNEL IN SHG-44 GLIOMA CELL-LINE

作　　者：王帅[1] 焦保华[1] 

机构地区：[1]河北医科大学第二医院神经外科,河北石家庄050000

出　　处：《中国应用生理学杂志》2009年第2期207-210,共4页Chinese Journal of Applied Physiology

摘　　要：目的:研究他莫昔芬对SHG-44胶质瘤细胞钠通道电流的作用。方法:采用全细胞膜片钳方法记录SHG-44细胞的钠通道电流,并观察施用不同浓度的他莫昔芬后电流的变化。结果:该钠通道电流特性为内向电流、快速激活失活,他莫昔芬能够明显阻断该电流,该阻断具有剂量依赖性及电压依赖性。在0 mV时,8μmol/L他莫昔芬对钾电流抑制率为69%。半数抑制浓度(IC50)为5.54μmol/L。结论:他莫昔芬可明显阻断SHG-44胶质瘤细胞上的钠通道,这可能是他莫昔芬抑制胶质瘤细胞增殖的机制之一。Aim： To explore the effect of tamoxifen on voltage-dependent sodium channels in SHG--44 glioma cell line. Methods： Whole-cell patch clamp technique was used to record the Na currents in SHG-44 cell line and to investigate the effect of tamoxifen of different concentration on this channel currents. Results： This channel activated and inactivated quickly. Tamoxifen could significantly decrease the amplitude of Na currents of SHG-44 cell line. This block effect was dose dependent and voltage dependent. When the holding potential was 0 mV, 8 μmol/L tamoxifen could block this currents 69%. The half inhibition concentration（IC50） was 5.54/maol/L. Conclusion： Tamoxifen could significantly block the voltage dependent sodium channel in malignant glioma cell line SHG-44. It might be one of the mechanisms that tamoxifen inhibit glioma proliferation.

关 键 词：SHG-44胶质瘤细胞 钠通道 膜片钳技术 

分 类 号：R730.23[医药卫生—肿瘤]