左肾动脉缩窄诱导心肌肥大大鼠心肌蛋白差异表达研究  

PROTEOMIC ANALYSIS OF MYOCARDIAL HYPERTROPHY INDUCED BY LEFT KIDNEY ARTERY COARCTATION IN RATS

作　　者：吕远远[1] 孙飙[1] 马继政[2] 

机构地区：[1]南京体育学院运动人体科学系,江苏南京210014 [2]解放军理工大学军事教育训练系,江苏南京210000

出　　处：《中国应用生理学杂志》2009年第2期237-242,共6页Chinese Journal of Applied Physiology

基　　金：江苏省体育局基金资助项目(TY6109)

摘　　要：目的:应用蛋白质组学方法分析病理性心肌肥大心肌细胞蛋白表达特征。方法:雄性SD大鼠16只,分为2组(n=8):两肾一夹组(2K1C)和假手术组(SO),术后饲养8周,使用普通多普勒和组织多普勒鉴定动物模型,然后提取心肌总蛋白,应用二维凝胶电泳技术,建立分辨率高和重复性良好的凝胶图像,质谱(MALDI-TOF-MS)鉴定差异蛋白点,与网络数据库进行匹配,并对鉴定蛋白进行分类。结果:两肾一夹大鼠心肌细胞有21个蛋白点表现出显著增加或减少,经数据库匹配,获得14种差异表达的蛋白质。结论:两肾一夹大鼠心肌出现一些差异表达蛋白质,它们可能在病理性心肌肥大的发生中起重要作用。To identify the expression of proteins in cardiomyocytes in rats with left kidney artery coarctation. Methods： 16 male SD rats were separated into 2 groups（ n = 8） ： 2 kidney 1 Clip group（2K1C） and sham operation group（SO）. The postoperational 8th week, after examination by normal doppler and tissue doppler echocardiography, the extracted proteins from cardiomyocytes were isolated by two-dimensional gel electrophoresis with staining. The gel images were acquired by scanner and 2-DE analysis software. Different spots observed on two 2D gels were selected and identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry（MALDI-TOF-MS）. Results： Overall, 21 protein spots showed significant difference, and 14 out of which were identified. Conclusion： Kidney artery coactation-induced cardiac hypertrophy displays different expression of proteins in cardiomyocytes.

关 键 词：蛋白质组 二维电泳 质谱 心肌肥大 肾动脉缩窄 

分 类 号：R364.3[医药卫生—病理学]