RP-HPLC同时测定口服液中的咖啡酸和阿魏酸  

Simultaneous Determination of Caffeic Acid and Ferulic Acid in Oral Liquid by RP-HPLC

作　　者：孙静涵[1] 李念兵[2] 罗红群[1] 

机构地区：[1]西南大学化学化工学院,重庆400715 [2]发光与实时分析教育部重点实验室(西南大学),重庆400715

出　　处：《西南大学学报（自然科学版）》2009年第5期47-50,共4页Journal of Southwest University(Natural Science Edition)

基　　金：重庆市科委自然科学基金资助项目(CSTC-2007BB0367)

摘　　要：采用高效液相色谱法同时测定了口服液中的咖啡酸和阿魏酸含量，用Shim-pack VP—ODS柱（150mm×4．6mm，0．3μm），流动相：乙腈-3％的冰醋酸水溶液（体积比为11：89），流速：1．0mL／min，检测波长：321nm．咖啡酸和阿魏酸的浓度与峰面积呈良好的线性关系，其线性范围分别为0．126～63．0／μg／mL（r=0．9997）和0．120-24．0μg／mL（r=0．9999），平均回收率（n=3）分别为100．7％和99．9％，相对标准偏差（RSD）分别为2．11％和2．12％，检出限分别为42ng／mL和43ng／mL，样品中咖啡酸和阿魏酸的平均含量分别为3．26μg／mL和2．36μg／mL．An HPLC method was established for the simultaneous determination of ferulic acid and caffeic acid in oral liquid. The Shim-pack VP-ODS column （150 min×4. 6 mm, 0. 3 μm） was used with a mobile phase of acetonitrile -3% acetic acid （11 ： 89 by volume）. The contents of ferulic acid and caffeic acid were detected at a wavelength of 321 nm. The flow rate was 1.0 mL/min and the injection volume was 20 μL. The calibration curve was linear in the range of 0. 126--63.0 μg/mL （r=0. 999 7） for caffeic acid and 0. 120--24.0 μg/mL （r=0. 999 9） for ferulic acid. The average recovery （n=3） was 100. 7% with RSD 2.11%/00 for caffeic acid and was 99.9% with RSD 2.12% for ferulic acid. The detection limit was 42 ng/mL for caffeic acid and 43 ng/mL for ferulic acid. The average content of the samples was 3.26μg/mL for caffeic acid and 2.36 μg/mL for ferulic acid.

关 键 词：高效液相色谱 阿魏酸 咖啡酸 口服液 

分 类 号：O657.72[理学—分析化学]