ET-1+138A/-基因多态性与女性舒张压的相关性  

Endothelin-1 Gene +138A/-Variant Association with Diastolic Blood Pressure in Female Hypertensive

作　　者：王珍[1] 吴志红[2] 杭丽玮[1] 窦贵旺[2] 蔡家春[2] 钟望涛[3] 徐明鸣[4] 周建中[1] 

机构地区：[1]重庆医科大学附属第一医院心内科,重庆400016 [2]重庆医科大学公共卫生学院流行病学教研室,重庆400016 [3]广东医学院附属医院神经内科,广东湛江524001 [4]重庆医科大学附属第一医院神内科,重庆400016

出　　处：《西南大学学报（自然科学版）》2009年第5期111-116,共6页Journal of Southwest University(Natural Science Edition)

基　　金：重庆市科委自然科学基金资助项目(CSTC;2006BB5044);重庆医科大学校办课题资助项目(QD200535)

摘　　要：目的:探讨重庆市地区人群血管内皮素1基因(Endothelin-1,ET-1)K198N,+138A/-位点基因多态性(Sin-gle nucleotide polymorphisms,SNP)与原发性高血压(EH)的相关性.方法:采用引物特异性片段长度多态性(PCR-SSP)法,检测95例原发性高血压患者及155例血压正常者ET-1基因的K198N,+138A/-位点多态性.并从中抽取36例高血压患者,40例血压正常者,用放射免疫法测定其血浆内皮素的浓度.结果:女性高血压中+138A/-多态性与舒张压有关(p=0.004),4A4A基因型舒张压比3A3A型高16 mmHg(p=0.004),较3A4A型高15 mmHg(p=0.035).将4A4A与3A4A型合并后其舒张压较3A3A型高(p=0.041).而在女性对照组以及男性中均无此关系.未发现K198N位点基因型与血压之间的相关性.结论:女性EH患者中,4A型携带者舒张压更容易升高,4A等位基因可能是女性EH患者血压升高的一个危险因素.进一步探讨其作用机制,可以为EH患者的个体化预防治疗提供依据.Objective： To investigate the association between K198N, ＋138A/- polymorphisms （SNP） of endothelin-1（ET-1） gene and essential hypertension in a sub-population of Chongqing. Methods： Sequence-specific primer polymerase chain reaction （SSP-PCR） was used to detect the genetic polymorphisms of endothelin-1 （ET-1） gene in 95 hypertensive patients and 155 normotensive subjects. Plasma ET concentration was measured by radioimmunoassay in 36 hypertensive patients and 40 normotensive subjects. Result： ＋ 138A/-gene variants influenced diastolic blood pressure （DBP） only in female hypertensive （p= 0. 004）, DBP in female individuals with 4A4A carriers of ＋ 138A/- was 16ram Hg higher than that in 3A3A carriers（p=0.041）and 15ram Hg higher than that in 3A4A carriers （p=0.035）. The above relationship was not found in the female control group and in the male groups and there was no relationship between K198N genotype and blood pressure. There was also no significant genotype-associated difference in plasma ET. Conclusion. Female hypertensive with 4A allele had a higher probability of having higher DBP; therefore 4A allele might be a risk factor for blood pressure rise in female hypertensive. Understanding this mechanism of action can further help to find new therapies for hypertensive patients, especially in the female population.

关 键 词：内皮素-1 基因多态性 原发性高血压 

分 类 号：R743.3[医药卫生—神经病学与精神病学]