微生物修复菌剂对复垦金尾矿土壤微生物群落的影响  

Effects of Microbial Remediation Inocula on Microbial Community in Gold-tailings Soil with Secondary Tillage

作　　者：李自刚[1] 彭爱娟[1] 屈凌波[2] 

机构地区：[1]郑州牧业工程高等专科学校,河南郑州450011 [2]河南工业大学,河南郑州450008

出　　处：《湖南农业科学》2009年第5期46-49,共4页Hunan Agricultural Sciences

基　　金：河南省重点科技攻关项目(0224660012)

摘　　要：对河南省灵宝市阳平镇文西渠金尾矿区麦冬草(Ophiopogogon japonicus(L.f.)Ker-Gawl.)复垦后的土壤细菌数量和群落的功能多样性进行了研究。结果表明复垦后金尾矿区土壤微生物细菌数达3.2×107个/(g土),并且文西渠金尾矿区土壤中存在一定量耐Au2+、Cd2+、Zn2+、Cr6+、Pb2+、Ni2+的菌株,主要是芽孢杆菌属(Bacillus)、假单胞菌属(Pseudomonas)和短杆菌属(Brevibacterium)。BIOLOGTM MicroPlate测试结果显示:Au、Cd重金属离子的复合污染使土壤微生物群落丰富度降低;外源耐Au、Cd的土壤ZZMZ微生物修复菌剂的添加可提高微生物对单一碳源底物的利用能力和增加利用不同碳底物的微生物数量,从而减轻重金属污染引起的金尾矿区土壤微生物群落功能多样性的下降,为尾矿区生态环境加速恢复提供了一条有效途径。Microbial activity and microbial community diversity in gold-tailings soils with secondary tillage were investigated, which were cultivated by Ophiopogogon japonicus （L.f.） Ker-Gawl in Wenxiqu Mining Area （Yangping, Lingbao, Henan, China）. The results showed that soil bacteria number reached to 3.2×10^7 per gram soil. There were a lot of bacteria could tolerant to Au^2＋, Cd^2＋, Zn^2＋, Cr^2＋, Pb^2＋, Ni^2＋, mainly belong to Bacillus, Pseudomonas and Brevibacterium. BIOLOGTM MicroPlate tests showed that community richness decreased in mine soils after polluted by Au and Cd ions, but microbial community metabolic profiles in mine soils had significant change after added microbial remediation inocula - ZZMZ. ZZMZ could improve the utilization ability of microbe on mono-carbon substrates and increase the quantity of microbe, which utilized different carbon substrates, thereby increased community richness, and provided an effective method for remediating environment in gold-tailings.

关 键 词：金尾矿 AU CD污染 耐Au Cd菌剂 土壤微生物群落功能多样性 

分 类 号：X172[环境科学与工程—环境科学]