纳米细菌致Ⅲ型前列腺炎的实验研究  

Nanobacteria induces Type Ⅲ prostatitis in rats

作　　者：明爱民[1] 沈学成[1] 周占松[1] 宋波[1] 

机构地区：[1]第三军医大学西南医院全军泌尿外科研究所,重庆400038

出　　处：《第三军医大学学报》2009年第11期1005-1008,共4页Journal of Third Military Medical University

基　　金：国家自然科学基金(30672094)~~

摘　　要：目的研究纳米细菌(nanobacteria,NB)对大鼠前列腺的慢性致炎作用。方法成年雄性SD大鼠40只,分为3组:对照组、模型组、四环素治疗组,分别为16、16、8只。模型组和四环素治疗组由尿道逆行注入NB建立大鼠动物模型。4周后,对照组、模型组各处死8只动物,观察前列腺组织中白细胞、卵磷脂小体及病理变化。治疗组用四环素灌胃治疗,其余动物用蒸馏水灌胃,连续4周,处死剩余动物,进行同样的观察。并将所有动物前列腺组织进行NB再分离、培养和鉴定。结果同对照组比较,4周后所有模型组动物前列腺组织呈慢性炎症改变,白细胞数明显升高,卵磷脂小体数明显下降(P<0.05)。8周后同模型组比较,治疗组动物炎症明显减轻,白细胞数明显下降,卵磷脂小体数明显升高(P<0.05)。模型组16只动物NB培养均阳性,对照组均阴性。结论NB能引起大鼠前列腺慢性炎症,NB可能是Ⅲ型前列腺炎的病因。四环素抗NB治疗有效。Objective To investigate the chronic inflammation causing effect of nanobacteria （NB） on the prostates of SD rats, and to provide experiment evidence for etiology of type m prostatitis. Methods Forty adult, male rats were divided into model group （16 rats）, treatment group （8 rats） and control group （16 rats） at random. The rats in model group and treatment group were reproduced by infusing NB suspension transurethrally, and the animals of control group received an infusion of normal saline. After 4 weeks, WBC, lecithine and pathological changes of prostates were observed in model group （8 rats ） and control group （8 rats）. Tetracycline （100 mg·kg^-1· d^-1） was administered to treatment group, and distilled water were given to other groups respectively for 4 weeks. Then all rats were sacrificed, and the same detections were done as before. NB from prostates of animal were re-isolated, cultured and identified. Results After 4 weeks, chronic infammation were observed in model group. WBC became significantly higher and lecithine of prostates were lower in model group than in control group （P 〈 0.05 ）. After 8 weeks, relieved chronic inflammation, decreased WBC and increased lecithine of prostates were observed in treatment group comparing with model group. There was significant difference in model group with control group and treatment group （P 〈 0.05 ）, and no difference was seen between control group and treatment group（ P 〉 0.05 ）. The positive cases of NB were 16 in model group, and none in control group. Conclusion Nanobacteria causes chronic inflammation in SD rat prostates. NB maybe the etiological agent of type Ⅲ prostatitis. Anti-NB treatment by tetracycline is effective for this inflammation.

关 键 词：纳米细菌 慢性前列腺炎 大鼠 四环素 

分 类 号：R-332[医药卫生] R378