五味子藤茎中多糖的提取及含量测定  

Extraction and Content Determination of Polysaccharide in the Rattan Stem of Schisandra chinensis

作　　者：胡彦武[1] 李婷婷[1] 

机构地区：[1]通化师范学院制药与食品科学系,吉林通化134002

出　　处：《安徽农业科学》2009年第15期6990-6991,共2页Journal of Anhui Agricultural Sciences

基　　金：吉林省教育厅"十一五"规划项目(吉教科合字[2008]第216号)

摘　　要：[目的]提取并测定五味子藤茎中多糖的含量。[方法]采用超声技术提取、苯酚一硫酸法测定含量，以葡萄糖（105℃干燥至恒重）为对照品。[结果]在10．125～71．064μg/ml的范围内，样品的浓度与吸光度呈良好线性关系，线性回归方程为A=0．0094C＋0．0029（r=0．9998），加样回收率为99．93％（n=5，RSD=0．06％），五味子藤茎中多糖平均含量为5．27％。[结论]五味子藤茎中多糖含量较高，具有较高的药用价值和开发前景。[ Objective] The aim of the research was to extract and determine the content of polysaccharide in the rattan stem of Schisadlra chinensis. [ Method] Polysaccharide was extracted by ultrasonic technology and the content was determined by phenol-sulfuric acid method, with glucose as the control substance. [Result] The sanole concentration has a good linear relationship with the absorbance within the range of 10.125 - 71.064 w,/ml.And the linear regression equation was A =0.009 4C ＋0.002 9（r =0.999 8）. The average recovery was 99.9＂3% （n = 5,RSD = 0106% ） and the average content of polysaccharide was 5.27%. [ Conclusion] The content of polysaccharide in the rattan stem of Schisandra chinensis was high, and it had higher medical value and development foreground.

关 键 词：五味子藤茎 多糖 苯酚-硫酸法 含量测定 

分 类 号：TQ223.121[化学工程—有机化工] TS201.3[轻工技术与工程—食品科学]