冻干和辐照对同种异体血管免疫原性的影响  

The effects of Frozen-dring plus irradiation on the immunogenicity of vascular allograft.

作　　者：薛莲[1] 张培华[1] 

机构地区：[1]上海第二医科大学附属第九人民医院血管外科研究室,200011

出　　处：《中华实验外科杂志》1998年第4期341-342,共2页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目(No.39270653)

摘　　要：目的探讨冻干和辐照处理对同种异体血管免疫原性的影响。方法以两个近交系大鼠间同种异体血管移植为模型,比较新鲜和制备血管移植后宿主细胞和体液免疫状态的变化,以及移植血管的病理变化。结果与新鲜的同种异体血管相比,处理血管移植后不引起宿主的急性排斥反应。结论冻干加辐照处理可明显降低同种异体血管的免疫原性,移植后不引起宿主的急性排斥反应。Objective To investigate the effects of Frozen-dring plus irradiation on the immunogenicity of vas-cular allograft. Methods Complement dependent cytotoxicity (CDC), mixed lymphocyte culture (MLC), and analysis of T cell sudsets as parameters, the model of vascular allograft for inbred rats of dif-ferent strains was used to study the changes in humoral and cellular immune responses of the host pre-and postprepared femoral arterial and venous transplant, and observe the pathological changes of trans-planted vascular allografts. Results The prepared allografts did not occur acute immune rejections con-trast to fresh allografts. However, the chronic inflammatory phenotype was found in the grafts. Conclu-sion Frozen-dring plus irradiation could obviously decrease the immunogenicity of vascular allografts. The prepared allografts could not induce acute rejection of the host.

关 键 词：同种异体移植 免疫原性 冻干 血管移植 辐照 

分 类 号：R622.4[医药卫生—整形外科] R392.4[医药卫生—外科学]