基于基因芯片蝎毒多肽提取物对H22肝癌抑制作用的研究  

Analysis of Inhibitory Function of Polypeptide Extract from Scorpion Venom on H22 Hepatoma with cDNAMicroarray

作　　者：张月英[1] 王兆朋[1] 贾青[1] 王朝霞[1] 张维东[1] 崔亚洲[2] 

机构地区：[1]山东省医学科学院基础医学研究所山东省现代药物与技术重点实验室,山东济南250062 [2]山东省医药生物技术研究中心国家卫生部重点实验室,山东济南250062

出　　处：《现代生物医学进展》2009年第10期1828-1831,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金青年基金(30600846);国家自然科学基金资助项目(30873408)

摘　　要：目的:通过表达谱基因芯片研究蝎毒多肽提取物PESV对H22肝癌基因表达的影响,探讨其可能的药物作用机理。方法:建立H22肝癌皮下荷瘤模型,将60只昆明小鼠随机分为模型组、PESV组、Gemcitabine和TNP-40组,每组15只;无菌摘取皮下肿瘤组织,提取总RNA,通过反转录方法制备cDNA荧光探针,进行芯片杂交,然后对芯片进行图像扫描,最后用Imagene4.0软件包对荧光信号进行分析。结果:蝎毒多肽提取物PESV作用于荷瘤小鼠后,有127个基因表达下调,433个基因表达明显上调,其中35个基因与抗肿瘤血管生成药物TNP-470处理组下调基因一致,有40个与化疗药物Gemcitabine处理组下调基因一致。结论:蝎毒多肽提取物PESV可能同时具有细胞毒作用和抗血管生成作用双重作用,有望成为一种极具前景的肿瘤治疗药物。Objective： To investigate the anti-tumor functions and possible mechanism of polypeptide extract from scorpion venom （PESV） on H22 hepatoma with cDNA microarray method. Methods： After establishment of tumor animal model by inoculating H22 s. 60 Kunming mice wearing H22 hepatoma were divided into 4 groups randomly： the model group, PESV group, Gemcitabine group and TNP-40 group. Total RNA of the subcutaneous tumors was extracted using Trizol reagents, and cDNA-labeled fluorescence was synthesized by reverse transcription method. Total RNA preparations from the tumors of these animals were hybridized to Mouse_6 biochip gene chips. Then the array was scanned and the signals were analyzed after hybridization by Imagene 4.0. Results： Compared with control group, 127 genes of the 48000 probe sets were up-regulated by more than 2 folds and 433 genes were down-regulated in tumor tissue treated with PESV. These genes were associated with angiogenesis, cell adhesion, cell cycle, apoptosis, and so on. The datasets identified from PESV-treated group were partly similar to the genes expression regulated by anti-angiogenic agent TNP-740 and partly similar to the regulated genes in response to chemotherapeutics Gemcitabine. PESV extremely not only showed anti-antigenic response but also have chemotherapeutic effects. Conclusions： The results show that PESV may be a promising cytotoxic and anti-antigenic double-response agent for cancer.

关 键 词：肝癌 蝎毒 基因芯片 

分 类 号：R735.7[医药卫生—肿瘤]