微囊化儿茶素对环磷酰胺致免疫低下小鼠的免疫调节作用  

Immunoloregulation effect of microcapsulated catechin on a mouse model of cyclophosphamide-induced hypoimmunity

作　　者：彭仕友[1] 何小解[1] 易著文[1] 许自川[1] 党西强[1] 杨华彬[1] 

机构地区：[1]中南大学湘雅二医院儿科,湖南省长沙市410011

出　　处：《中国组织工程研究与临床康复》2009年第21期4123-4127,共5页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：湖南省自然科学基金资助项目(02JJXY3020);湖南省教育厅重点项目基金资助(02A015)~~

摘　　要：背景:儿茶素具有强抗氧化活性已成共识,但其对免疫调节作用报道甚少;同时,儿茶素存在不稳定性及脂溶性差等缺陷,微胶囊技术是解决这一问题的一种有效途径。目的:观察微囊化儿茶素与儿茶素对环磷酰胺致免疫低下小鼠特异性细胞免疫、特异性体液免疫、非特异性体液免疫及胸腺与脾脏脏器系数的影响。设计、时间及地点:随机对照动物实验,于2007-11/12在中南大学湘雅二医院医学实验动物中心完成。材料:安化小叶种儿茶素由湖南金农生物资源股份有限公司提供;儿茶素的微囊化由中南大学湘雅二医院小儿肾脏病研究室完成。环磷酰胺由上海华联制药有限公司生产。方法:600只昆明种小鼠随机分生理盐水组,儿茶素500,250,125mg/kg组,乙基纤维素500,250,125mg/kg组;微胶囊化儿茶素500,250,125mg/kg组,环磷酰胺组,环磷酰胺+微胶囊化儿茶素500,250,125mg/kg组,环磷酰胺+儿茶素500,250,125mg/kg组;环磷酰胺+乙基纤维素500,250,125mg/kg组共20组。生理盐水、儿茶素、乙基纤维素、微胶囊化儿茶素均灌胃给药,连续给药14d;环磷酰胺自第11天起腹腔注射给药80mg/kg,连续4d。主要观察指标:利用小鼠迟发型超敏反应测定特异性细胞免疫功能,血清溶血素实验测定特异性体液免疫功能,小鼠碳粒廓清指数测定非特异性体液免疫功能以及脾指数与胸腺指数衡量机体免疫功能。结果:与生理盐水组比较,微胶囊化儿茶素与儿茶素500,250mg/kg组的耳肿胀度明显增加;微胶囊化儿茶素500,250,125mg/kg组半数溶血值、碳粒廓清指数和吞噬指数、脾指数、胸腺指数均明显增高(P<0.01,P<0.05);儿茶素500mg/kg组小鼠的半数溶血值有一定的提高,儿茶素500,250,125mg/kg组小鼠的碳粒廓清指数和吞噬指数、脾指数、胸腺指数均明显提高(P<0.05,P<0.01)。与环磷酰胺模型组小鼠比较,环磷酰胺+儿茶素500,250mg/kg组及环磷酰胺+微胶囊BACKGROUND： It is well known that catechin has a strong antioxygen activity; however, there are few reports about immunoloregulation. In addition, catechin is limited by instability and poor liposolubility. Microencapsulation is an effective way to solve the problems mentioned above. OBJECTIVE： To compare the effect of microcapsulated catechin and single catechin on specific cellular immunity, specic humoral immunity, non-specific humoral immunity, and organ coefficient of thymus and spleen in a mouse model of cyclophosphamide-induced hypoimmunity. DESIGN, TIME AND SETTING： A randomized controlled animal study was performed at Medical Experimental Animal Center, the Second Xiangya Hospital of Central South University from November to December 2007. MATERIALS： Catechin was provided by Jinnong Biotic Resource Co., Ltd., Hunan; microcapsulated catechin was provided by Institute of Pediatric Renal Disease, the Second Xiangya Hospital of Central South University; cyclophosphamide was provided by Hualian Pharmaceutical Co., Ltd., Shanghai. METHODS： A total of 600 Kunming mice were randomly divided into saline group, catechin （500, 250, 125 mg/kg） groups, ethyl cellulose （500, 250, 125 mg/kg） groups, microcapsulated catechin （500, 250, 125 mg/kg） groups, cyclophosphamide group, cyclophosphamide ＋ microcapsulated catechin （500, 250,125 mg/kg） groups, cyclophosphamide ＋ catechin （500, 250, 125 mg/kg） groups, and cyclophosphamide ＋ ethyl cellulose （500, 250, 125 mg/kg） groups. Saline, catechin, ethyl cellulose, and microcapsulated catechin were perfused respectively for 14 successive days; cyclophosphamide （80 mg/kg） was intraperitoneally injected from the 1 lth day for 4 successive days. MAIN OUTCOME MEASURES： Specific cellular immune function was detected using delayed hypersensitivity; specific humoral immune function was measured using serum-hemolysin test; non-specific humoral immune function and organ coefficient of spleen and thymus were detected using carbon e

关 键 词：儿茶素 微囊 免疫 环磷酰胺 小鼠 

分 类 号：R318[医药卫生—生物医学工程]