三七总皂苷对快速老化模型小鼠脑内syp，tau基因表达的影响  

Effect of Panax notoginseng saponins on syp and tau gene expression in brain of senescence accelerated mouse

作　　者：吕良[1] 钟振国[1] 吴登攀[1] 柴立民[2] 张雯艳[1] 

机构地区：[1]广西中医学院新药研究开发中心,广西南宁530001 [2]中国中医科学院眼科医院,北京100040

出　　处：《中国中药杂志》2009年第10期1261-1263,共3页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(30560189);广西高校人才小高地建设创新团队资助计划

摘　　要：目的:研究三七总皂苷(Panax notoginseng saponins,PNS)对快速老化模型小鼠(senescence accelerated mouseprone 8,SAMP 8)脑内突触素(synaptophysin,syp)、tau基因表达的影响。方法:SAMP 8小鼠随机分为PNS低、高(23.38,93.50mg.kg-1.d-1)剂量组、阳性对照药物石杉碱甲(0.038 6 mg.kg-1.d-1)组、模型组。各给药组分别按设定剂量灌胃给药,空白组灌服双蒸水,连续8周。应用实时荧光逆转录聚合酶链式反应(real-time RT-PCR)的方法,检测PNS对SAMP 8脑内syp,tau mRNA含量的影响。结果:PNS高、低剂量组均可以增加syp mRNA含量(P<0.05或P<0.01),PNS对tau mRNA含量未见明显影响。结论:PNS可以在转录水平上调SAMP 8脑内syp基因的表达。Objective： To study the effect of Panax notoginseng saponins （PNS） on （synaptophysin, syp） and tau gene expression in the brain tissue in senescence accelerated mouse prone 8 （SAMP 8 ）. Method： SAMP8 were randomly divided into 4 groups： PNS 23.38, 93.50 mg ·kg^-1 group, huperzin A 0. 038 6 mg · kg^-1 · d^-1 group and blank control group; the drug groups were treated with the designed drugs respectively per day by intragastric administration for 4 consecutive weeks, and double distilled water was given to blank control group. After treatment, the mRNA content of tau and syp were assayed by reverse transcription （RT） and real-time polymerase chain reaction （real-time PCR）. Result： Compared with blank control group, the syp mRNA contents were increased in PNS groups （P 〈0. 05 or P 〈 0. 01 ） , and the tau mRNA content were not significant difference in all groups. Conclusion ： This study suggests that PNS can up-regulate syp gene expression at transcriptional level in the brain of SAMP 8.

关 键 词：三七总皂苷 快速老化模型小鼠 突触素 TAU 基因表达 

分 类 号：R285.5[医药卫生—中药学]