密骨胶囊和健脾方对切卵大鼠小肠及腓肠肌中VDR mRNA表达的影响  

Influence of Migu(密骨)capsule and Strengthening Spleen prescription(健脾方) on the expression of small intestine VDR mRNA in calf muscle on ovariectomy rats

作　　者：王立童[1] 赵咏芳[1] 王翔[1] 徐宇[1] 陈元川[1] 詹红生[1] 

机构地区：[1]上海中医药大学附属曙光医院上海市中医药研究院骨伤科研究所,上海200021

出　　处：《中国骨伤》2009年第5期367-370,共4页China Journal of Orthopaedics and Traumatology

基　　金："中医骨伤科学"国家重点学科和上海市重点学科项目(编号:100508;T0303);国家自然基金(编号:30371793;30300459;30472222;30500674);教育部新世纪优秀人才支持计划(编号:NCET040438);教育部博士点基金(编号:20040268012);上海市曙光计划(编号:03SG61);上海市科委重点项目(编号:04JC14071);上海市科委启明星计划(编号:04qmx1434)

摘　　要：目的:观察密骨胶囊、健脾方对大鼠小肠、腓肠肌中维生素D受体(vitamin D receptor,VDR)mRNA表达的影响,探讨补肾、健脾不同功效的中药复方,是否对机体不同组织器官VDR mRNA的表达存在一定选择性。方法:复制切卵大鼠骨质疏松模型,随机分为假切组、模型组、密骨胶囊组和健脾方组,术后12周开始灌胃,灌胃12周后取材。测定骨密度、子宫重量、腓肠肌重/体重,同时对大鼠小肠、腓肠肌中VDR mRNA进行半定量。结果:密骨胶囊能提高切卵大鼠骨密度(P<0.05),提高腓肠肌重/体重(P<0.05),上调小肠和腓肠肌中VDR mRNA的表达(P<0.01);健脾方能维持大鼠骨密度(P>0.05),上调腓肠肌中VDR mRNA的表达(P<0.01);密骨胶囊、健脾方均无增加子宫重量作用。结论:密骨胶囊和健脾方能通过上调切卵大鼠小肠和腓肠肌中VDR mRNA的表达,而起到治疗骨质疏松症的作用。Objective： To observe the effect of Migu capsule and Strengthening Spleen prescriptions on the expression of vitamin D receptor on small intestine and calf muscle of the rat, while observing whether the Chinese medicine complex prescriptions of different effect such as invigorating the kidney and strengthening the spleen had selectivity to expression of the VDR mRNA. Methods：Copy the model of osteoporosis by ovariectomy operation,the rats were randomly divided into four groups, pseudo-resection group, model group, Migu capsule group and strengthening Spleen prescriptions group. Drug delivery 12 weeks later from operation, stomach lavage last for 12 weeks. Then, to observe the effect of medicine on the rats＇ bone mineral density, uterus weight, calf muscle/weight, and the VDR mRNA in small intestine and calf muscle through RT-PCR. Results： Mig, capsule enhanced bone mineral density （P〈0.05）, raised calf muscle/weight （P〈0.05）, up-regulated expression of calf muscle VDR mRNA in the small intestine （P〈0.01）. Strengthening Spleen prescriptions remained bone mineral density （P〉 0.05）, up-regulated expression of calf muscle VDR mRNA （P〈0.01）, there was no obvious difference in uterus weight in Migu capsule group and Strengthening Spleen prescription group. Conclusion：Migu capsule and Strengthening Spleen prescriptions can cure osteoporosis by improving the expression of calf muscle VDR mRNA in the small intestine.

关 键 词：密骨胶囊 健脾方 卵巢切除术 大鼠 小肠 腓肠肌 维生素D RNA 信使 基因表达 

分 类 号：R734.2[医药卫生—肿瘤] R274.91[医药卫生—临床医学]