从血细胞快速抽提DNA的方法探讨  

Access Study of Rapid DNA Extraction from Blood Cells

作　　者：段勇[1] 王玉明[1] 赵淮[1] 宋滇平[1] 张秋霞[1] 刘华[1] 

机构地区：[1]昆明医学院第一附属医院检验科

出　　处：《昆明医学院学报》1998年第2期28-30,共3页Journal of Kunming Medical College

基　　金：云南省科委和国家人事部非教育系统留学归国人员科技活动资金

摘　　要：在提取白细胞ＤＮＡ经典方法的基础上，改进从血细胞快速抽提ＤＮＡ的方法．应用２０％ＴｒｉｔｏｎＸ－１００为溶血剂，以酚／氯仿／异戊醇混合液为抽提剂，能从３ｍＬ的全血中提取６０～９０μｇ的优质ＤＮＡ，具有快速、经济。Based on the classical protocol to isolate DNA from leukocytes, improvement was made for rapidly extracting DNA from blood cells. With 20% of triton X-100 as hemolytic agent and mixture of phenol/chloroform/isoamyl alcohol as extraction agent, the method yield 60～90 μg DNA of fine quality from 3 ml blood, demonstrating such fine advantages as rapidity, economy and handling at normal temperature.

关 键 词：白细胞 DNA 提纯 基因组 

分 类 号：R394－33[医药卫生—医学遗传学]