脊索瘤细胞系CM-319中放化疗抵抗相关基因的表达及临床意义  

Expression of resistance genes against chemotherapy and radiotherapy and the clinical significance in chordoma cell line CM-319

作　　者：纪振钢[1] 龙华[1] 张殿忠[1] 裘秀春[1] 孙宏慧[1] 马琼[1] 刘云燕[1] 文艳华[1] 王育才[1] 范清宇[1] 

机构地区：[1]第四军医大学唐都医院全军骨肿瘤研究所,陕西西安710038

出　　处：《大连医科大学学报》2009年第3期237-241,共5页Journal of Dalian Medical University

基　　金：国家自然科学基金重点项目(30330610);国家自然科学基金面上项目(30873027);陕西省科学技术研究发展计划项目(2008K09-09)

摘　　要：[目的]探讨多药耐药相关基因如缺氧诱导因子1α(HIF-1α)、多药耐药基因(MDR1)及多药耐药相关蛋白(MRP1)在脊索瘤细胞系CM-319中的表达及临床意义。[方法]用RT-PCR检测HIF-1α、MDR1、MRP1基因表达,间接免疫荧光及免疫细胞化学Envision法检测HIF-1α、MRP1蛋白在CM-319中的表达情况,并分析其与放化疗抵抗的关系。[结果]常氧条件下,在脊索瘤细胞系中能检测到HIF-1α及MRP1 mRNA的表达,但未检测到MDR1 mRNA的表达(P<0.01),间接免疫荧光及免疫细胞化学可以检测到HIF-1α表达在细胞浆及核中,而MRP1主要表达在细胞浆中。[结论]HIF-1α与MRP1的过度表达在脊索瘤的放化疗抵抗中可能具有重要作用,而MDR1表达未被检测到,提示脊索瘤的放化疗抵抗主要与HIF-1α和MRP1相关。[ Objective ] To investigate the expression of some genes related to resistance to chemotherapy and radiotherapy, such as HIF- 1α, MRP1 and MDR1 , and their clinical significance in chordoma cell line CM -319. [ Methods] The expression of HIF - 1α, MDR1 and MRP1 was detected with reverse transcription polymerase chain reaction （ RT - PCR） and indirect immunofluorescence staining and immunocytochemical technique （ChemMateTM Envision）. The relationship between HIF -1α, MRP1 and resistance to chemotherapy and radiotherapy was statistically analyzed. [ Results ] The positive expression of HIF-1α and MRP1 was detected in CM -319 in normal Oxygen conditions, while MDR1 was not detected by RT- PCR（P 〈0.01 ）. The positive expression of HIF- 1α protein was detected in cytoplasm and nucleus, and MRP1 mostly in cytoplasm with indirect immunofluorescenee staining and immunocytochemieal technique. [ Conclusions ] The overexpression of HIF - 1α, MRP1 plays an important role in the resistance to chemotherapy and radiotherapy of chordoma cell line. And the expression of MDR1 was not detected. It may be a signature that the resistance to chemotherapy and radiotherapy was related with HIF - 1α, MRP1 in chordoma cell line.

关 键 词：缺氧诱导因子1Α 多药耐药基因1 多药耐药相关蛋白1 脊索瘤 

分 类 号：R739.4[医药卫生—肿瘤]