核心家系5-羟色胺转运体基因第二内含子多态性与自杀未遂的关联分析  

An analysis on the relationship between polymorphism of intron 2 in serotouin transporter gene and attempted suicide in center families

作　　者：高新学[1] 况利[1] 艾明[1] 李大奇[1] 楼丹丹[1] 刘婉婷[1] 赵正中[1] 

机构地区：[1]重庆医科大学附属第一医院心理卫生中心,重庆100016

出　　处：《中华行为医学与脑科学杂志》2009年第2期105-107,共3页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：重庆市卫生局资助项目（07-2-049）

摘　　要：目的探讨5-羟色胺转运体（5-HTT）基因第二内含子多态性（5-HTTVNTR）位点与自杀未遂的关联。方法收集来自重庆地区汉族人群中，以自杀未遂核心家系作为研究对象，在86个核心家系的258个家系成员中应用聚合酶链反应（PCR）和限制性片段长度多态性方法，对5-HTTVNTR位点多态性与自杀未遂之间的分子遗传学联系进行了以家系为基础的基于单体型的单体型相对风险分析及连锁不平衡分析。结果基因型10／10、10／12、12／12在患者组和父母组分别为：13，32，41和21，89，62和等位基因频率10，12在患者组和父母组分别为：58，144和131，213均在自杀未遂患者组和父母组之间差异无显著性（Х^2=4．892，P=0．0867；Х^2=0．939，P=0．332）。未发现5-HTTVNTR位点多态性与自杀未遂有关联（X2=2．77，P〉0．05），多等位基因TDT统计分析也未发现存在连锁不平衡（Х^2=1．90，P〉0．05）。5-HTTVNTR位点仅扩增出12和10拷贝两种形式的等位基因片段。结论汉族人群中5-HTTVNTR位点多态性与自杀未遂间没有明显相关性。Objective To explore the association between attempted suicide and polymophism of intron 2 in serotonin transporter （5-HIT）. Methods The study selected Chongqing Han center families who attempted suicide to be research subjects. Genomie DNA was isolated from 86 core pedigrees, sum to 258 family members. The genotype was carried out using polymerase chain reaction（PCR） and restriction fragment length polymorphisrn techniques. A family-based link-age relative risk based on haplotype and disequilibrium analysis were conducted. Results The genetype 10/10, 10/12 and 12/12 were respectively 13,32,41 and 21,89,62 in respectively in patient group and parent＇s. The frequency of 5-HTTVNTR gene 10,12 were respectively 58,14 and 13,23 in patient group and parent, which was no significant difference between patient group who had attempted suicide and parent （ Х^2 = 4.892, P = 0.0867 ; Х^2 = 0.939, P = 0. 332）. The HHRR（ Х^2 = 2.77, P 〉 0.05 ） and the TDT（ Х^2 = 1.90, P 〉 0.05 ） of multiple allele did not show association between polymorphism of 5-HTTVNTR and attempted suicide. The polymorphisrn of 5-HTrVNTR gene was only amplified two forms of allele fragment 12 and 10. Conclusion It is no obviously association between polyrnorphisrn of 5-HTTVNTR gene and attempted suicide.

关 键 词：自杀未遂 基因 多态现象(遗传学) 5-羟色胺转运体 

分 类 号：R686[医药卫生—骨科学]