CD44v6修饰DC融合瘤苗抗胰腺癌作用的研究  

Antitumor effect of fusion hybrid of dendritic cells expressing CD44V6

作　　者：曾波[1] 刘红专[1] 蔡华容[1] 李丽玲[1] 

机构地区：[1]绵阳市第三人民医院消化内科,四川绵阳621000

出　　处：《现代医药卫生》2009年第11期1603-1605,共3页Journal of Modern Medicine & Health

摘　　要：目的:探讨CD44v6修饰树突状细胞(DC)融合瘤苗抗胰腺癌的作用,为肿瘤免疫治疗提供实验依据及理论基础。方法:(1)取小鼠骨髓细胞在细胞因子GM-CSF、IL-4、TNF-α的诱导下体外大量扩增DC细胞;(2)在PEG的作用下,进行DC和小鼠胰腺癌细胞(TD2)的融合,并利用HAT/HT系统筛选出纯净融合细胞株;(3)Lipofectamine2000的介导下,将pBud-CD44v6转染到肿瘤融合细胞中表达;(4)荷瘤小鼠接种融合瘤苗,测量肿瘤大小。结果:成功构建了CD44v6修饰的DC/TD2融合瘤苗;免疫组肿瘤体积明显小于对照组。结论:小鼠骨髓来源的DC在PEG作用下成功与TD2细胞融合,同时以CD44v6加以修饰,且融合细胞可有效诱导T淋巴细胞产生强大抗胰腺癌作用。Objective ：To study the antitumor effect of fusion hybrid of dendritic eells（DC） and pancreatic cancer cell TD2 expressing CD44v6 for providing the experimental and theoretical foundation on tumor immunotherapy. Methods：（1）Bone marrow-derived mice DCs were largely expanded with cytokine of GM-CSF, IL-4 and TNF-α; （2）DCs and TD2 ceils were fused by polyethylene glyeal（PEG）, and the completely fused ones were screened by HAT/HT system ;（3）The recombinant pBud-CD44v6 vector was transfected into the fused cells （hybridoma cell） by lipofectamine 2000, and identhqed by RT-PCR;（4）Fused cells were implanted to bearing--cancer mice, and the volume of tumors were studied. Results：DC/TD2 fused cell vaccine modified with CD44v6 was successfully constructed;the volume of tumors of immune group was smaller than that of control ones. Conclusion：Mice bone-marrow derived DC cells can be fused with TD2 cells by PEG, and the fused ceils can induce strong antitumor immune effect of T lymphocyte.

关 键 词：CD44V6 树突状细胞 细胞融合 胰腺癌 

分 类 号：R73[医药卫生—肿瘤]