5-羟色胺受体与调节性T细胞在抑郁症免疫功能紊乱中的关联作用  

The correlation of 5-HT1a receptor and the regular T cell in the immune imbalance of depression

作　　者：李艺[1] 杨欢[1] 肖波[1] 邱雯静[1] 杨乐[1] 吴志国[1] 余庆皋[1] 

机构地区：[1]中南大学湘雅医院神经内科,长沙410008

出　　处：《中华行为医学与脑科学杂志》2009年第4期308-310,共3页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：湖南省教育厅资助项目（07D080）

摘　　要：目的观测抑郁症患者免疫失衡的特征，从5-羟色胺受体（5-HT1aR）和调节性T细胞表达变化的关系，探讨抑郁症免疫失衡的可能机制。方法采集27例抑郁症患者外周血，利用ELISA方法测定血清细胞因子IL-2，IL-10，TGF-β1浓度，逆转录-聚合酶链反应（RT-PCR）方法检测患者5-HT1aR和FoxP3的mRNA水平，免疫磁珠分离调节性T细胞，共聚焦显微镜观察调节性T细胞5-HT1aR受体和FoxP3的共表达，并与正常对照组进行对照。结果与正常对照组标本相比，抑郁症患者血清IL-2表达水平上调[（184．681±8．472）pg/ml，（82．845±12．292）pg／ml]，IL-10[（6．765±0．611）pg／ml，（9．593±0．921）pg／ml]，TGF-β1[（14．042±2．170）ng／ml，（20．981±3．98）ng／ml]的表达水平降低（均P〈0．01）；外周血中调节性T细胞数量减少[（13．139±4．587）10^7个，（20．583±3．484）10^7个]，单个核细胞5-HT1aR和FoxP3的mRNA表达水平降低（均P〈0．01）；同时通过共聚焦显微镜观察到调节T细胞上的5-HT1aR及FoxP3表达减弱。结论抑郁症患者中存在免疫失衡现象，5-HT1aR通过影响调节性T细胞在抑郁症患者免疫失衡的病理生理机制发挥着重要的作用。Objective To observe the immune imbalance in depression patients, as well as to explore the role of 5-HT ,5-HT1a receptor（ 5-HT1aR） and the regular T cell in the pathological imbalance mechanism of depression disorder. Methods 27 depression patients were selected with the criteria as CCMD-3 and DSM-IV. The concentration of serum IL-2,IL-10 ,TGF-[31 were detected by ELISA, the serum 5-HT concentration was measured by high performance liquid chromatogram, the 5-HT1,R and FoxP3 mRNA expression level are obtained by RT-PCR, CD4 ± CD25 ± T regular cells were detached from PBMCs by immunomaguetie beads and the coexpression of the 5- HTlaR and FoxP3 in regular T cells are observed by Confoeal Microscope. Results The serum concentration of IL-10 （6. 765 ± 0. 611 ） pg/ml VS （9. 593 ±0. 921 ） pg/ml and TGF-β1 （6. 765 ± 0.611 ） pg/ml VS （9. 593 ± 0. 921 ） pg/ml were decreased while the IL-2 （ 184.681 ± 8. 472 ）pg/ml VS （82. 845 ± 12. 292 ）pg/ml was increased in patients compared with those of controls （P〈 0.05 ）. Both 5-HT1aR and FoxP3 mRNA expression in immune cells and the proportion of regular T cells were decreased in patients, meanwhile the 5-HT1aR and FoxP3 expression in regu- lar T cells were also specially declined when observed by immunofluoreseenee method. Conclusion The results of this study support the hypothesis that the 5-HT plays a role in the immune imbalance of depression, probably through the regular T cell pathway via 5-HT1aR.

关 键 词：调节性T细胞 抑郁症 5-羟色胺受体 

分 类 号：R749.4[医药卫生—神经病学与精神病学]