辐照氟银脐动脉移植于兔颈动脉后内膜增生及ki-67表达研究  

Expression of ki-67 and intimal hyperplasia of the irradiated human umbilical artery incorporated with nofloxacin and silver grafts

作　　者：刘志平[1] 王坚[1] 朱宪明[1] 张玉龙[1] 李淑珍[1] 赵龙[1] 郭俊晓[1] 任杰[1] 高荣[1] 邱能庸[1] 

机构地区：[1]内蒙古医学院附属医院胸心外科,内蒙古呼和浩特010059

出　　处：《中国医师杂志》2009年第5期599-602,共4页Journal of Chinese Physician

基　　金：内蒙古自然科学基金资助项目（200607010901）

摘　　要：目的观察辐照氟银脐动脉移植到兔颈动脉后的内膜增生情况及ki-67的表达。为冠状动脉血管重建提供新的血管材料源。方法无菌条件下取人胎儿脐动脉20条制成辐照氟银脐动脉。30只兔的左、右两侧颈动脉同时分别植入辐照氟银脐动脉（实验组）和自体颈动脉（对照组），作为自身对照，术后2周和6周观察血管通畅情况，取材作大体、组织形态学和免疫组织化学观察，对吻合口通畅血管段内膜厚度及ki-67的阳性表达进行分析。结果（1）移植术后血管内皮细胞再生良好，总通畅率为89．6％。（2）光镜检查移植段血管壁三层结构均存在，管壁有少量单核及淋巴细胞浸润，内腔由内皮细胞覆盖。（3）移植术后2周实验组及对照组内膜厚度差异无统计学意义（P〉0．05），术后6周实验组的内膜增生比对照组重，但差异无统计学意义（P〉0．05）。（4）免疫组织化学检测表明实验组的ki．-67的表达较对照组高，术后2周2组差异无统计学意义（P〉0．05），术后6周差异亦无统计学意义（P〉0．05）。结论辐照氟银脐动脉的内膜增生与自体动脉差异无显著性，有望在冠状血管外科成为比较理想小口径血管移植物。Objective To investigate the expression of ki-67 and the development of the intimal hyperplasia（IH） of the irradiated human umbilical artery incorporated with nofloxacin and silver（IHUAINS） grafts into the carotid arteries of the rabbit. Methods Twenty IHUAINSs were sterilely produced. Thirty rabbit were performed bilateral carotid bypass grafting. The IHUAINS（ experimental group）and the left carotid arteries （.control group） were implanted in the left and right carotid arteries respectively. Graft patency was checked at the 2nd and 6th week after implantation, and the grafts were studied with standard histological techniques and immunohistochemical method for measurement Of intimal thickness and the expression of ki-67. Results The total patency rate of the grafts was 89.6%. Light microscopic examination of the grafts revealed intimal and media proliferation, cellular in-filtration. The endothelial cells covered the vascular lumen. There was no significant difference of the intimal thickness between two groups at the 2nd week after grafting （ P〉0.05）. The intimal thickness of the experimental group was larger than that in control group at the 6th week after implantation without statistical significance （ P〉0.05 ）. At the same time, immunocytochemical analysis showed that the expression of ki-67 in the experimental group was stronger than that in control group without statistical significance（ P 〉 0. 05）. Conclusion The IH of the IHUAINS was larger than that of the autologous artery, but there is no difference between these two groups. Thus, IHUAINS may be an ideal graft in the field of coronary surgery.

关 键 词：脐动脉/移彬辐射效应 颈动脉/外科学 血管内膜 KI-67抗原 银 氟 

分 类 号：R[医药卫生]