CPH肝组织、血清HBV与血清HBV标志物不同状态的相关性探讨  

A Study of Relativity Between HBV DNA in Sera and Hepatic Tissue and Different Condition of Five Items in Sera from patients with chronic Persistent Hepatitis

作　　者：洪丰[1,2,3,4] 白守国[1,2,3,4] 赵洪涛 孙文生[1,2,3,4] 

机构地区：[1]山东省济宁市第一人民医院 [2]山东省济宁市妇女儿童医院 [3]山东省立医院 [4]山东医科大学

出　　处：《胃肠病学和肝病学杂志》1998年第2期120-122,共3页Chinese Journal of Gastroenterology and Hepatology

摘　　要：目的和方法：经肝活检诊断为慢性迁延性肝炎（ＣＰＨ）５１例，均做ＰＣＲ、斑点杂交进行肝组织、血清ＨＢＶＤＮＡ检测，同时用ＥＬＩＳＡ方法检测了ＨＢＶ血清标志物。结果，ＨＢＶＤＮＡ检出率依次为肝组织ＰＣＲ９４１％（４８／５１）、血清ＰＣＲ９２２％（４７／５１）和肝组织斑点杂交９０２％（４６／５１）。三者之间无显著差异（χ２＝０４９５，Ｐ＞００５）。各项血清标志物总检出率ＨＢｓＡｇ３７２％（１９／５１），ＨＢｅＡｇ３３３％（１７／５１）和抗－ＨＢｅ２１５％（１１／１５），显著低于ＰＣＲ和斑点杂交ＨＢＶＤＮＡ的检出率（χ２＝３０．９，Ｐ＜０００５）。在抗－ＨＢｅ（＋）的１１例患者中１０例检出肝组织和血清ＨＢＶＤＮＡ，８例抗－ＨＢｓ（＋）患者，６例肝组织及血清ＰＣＲＨＢＶＤＮＡ阳性，结论：表明抗－ＨＢｅ（＋）及／或抗－ＨＢｓ（＋）不能代表ＨＢＶ复制停止或被清除。Abstract Aim and Methods:We used polymerase chain reaction(PCR) assay and slot-blot hybridization for detecting hepatitis B Virus (HBV) DNA in the serum and hepatic tissue of 51 patients with chronic persistent hepatitis(CPH) diagnosed by hepatic biopsy, while using ELISA to detect HBV five seral items. Results: The results showed that:of 94.1%(48/51) cases positive for HBV DNA by PCR of hepatic tissue, 92.2%(47/51) Cases positive for HBV DNA by PCR of serum and 90.2%(46/51) by slot-blot hybridization of hepatic tissue. No Significant differenciation among them (χ 2=0.495,P>0 05). the positive rates of HBV five seral items as follows:HBsAg 37.2%(19/51),HBeAg 33.3%(17/51) and antibody against HBV e antigen 21.5%(11/51), Significantly lower than the positive rate of HBV DNA by PCR and slot-bolt hybridization (χ 2=30.9,P<0 005). In 11 patients for antibody against HBV e antigen, 10 were positive for HBV DNA by PCR of serum and hepatic tissue, while in 8 patients positive for antibody against HBV surface antigen, 6 were positive. Conclusions: this indicated that positive for antibody against HBV e antigen and/or for antibody against HBV surface antigen couldn′t tell the cancelation or stop of replication of HBV, the hepatic disorder might still exist.

关 键 词：慢性迁延性 肝炎 斑点杂交 HBVDNA PCR 

分 类 号：R575.1[医药卫生—消化系统]